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However, if misregulated, deficiency or excess of copper ions can lead to several diseases. Therefore, it is important to have reliable methods to detect, monitor and visualise this metal in cells. Herein we report a new optical probe based on BODIPY, which shows a switch\u2010on in its fluorescence intensity upon binding to copper(I), but not in the presence of high concentration of other physiologically relevant metal ions. More interestingly, binding to copper(I) leads to significant changes in the fluorescence lifetime of the new probe, which can be used to visualize copper(I) pools in lysosomes of live cells via fluorescence lifetime imaging microscopy (FLIM).<\/jats:p>","DOI":"10.1002\/anie.202109349","type":"journal-article","created":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T09:15:17Z","timestamp":1628673317000},"page":"23148-23153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["Selective Detection of Cu\n                    <sup>+<\/sup>\n                    Ions in Live Cells via Fluorescence Lifetime Imaging Microscopy"],"prefix":"10.1002","volume":"60","author":[{"given":"Martin","family":"Priessner","sequence":"first","affiliation":[{"name":"Department of Chemistry Imperial College London  White City Campus London W12 0BZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter 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