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Hyperpolarization by dissolution dynamic nuclear polarization (D\u2010DNP) provided a <jats:sup>13<\/jats:sup>C signal enhancement between 3000\u20136000 for the ligand 4\u2010(trifluoromethyl) benzene\u20101\u2010carboximidamide binding to trypsin. The measurement of <jats:sup>13<\/jats:sup>C <jats:italic>R<\/jats:italic><jats:sub>2<\/jats:sub> relaxation dispersion was enabled without isotope enrichment, using a series of single\u2010scan Carr\u2013Purcell\u2013Meiboom\u2013Gill experiments with variable refocusing delays. The magnitude in dispersion for the spins of the ligand is correlated to the position with respect to the salt bridge between protein and the amidine group of the ligand, indicating the ligand binding orientation. 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