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A wide range of aldehydes and ketones can be used as acceptor substrates; however, the donor substrate range is mostly limited to achiral \u03b1\u2010keto acids and simple aldehydes. By using a unifying retro\u2010biosynthetic approach towards acyl\u2010branched sugars, we identified a subclass of (myco)bacterial ThDP\u2010dependent enzymes with a greatly extended donor substrate range, namely functionalized chiral \u03b1\u2010keto acids with a chain length from C\n                    <jats:sub>4<\/jats:sub>\n                    to C\n                    <jats:sub>8<\/jats:sub>\n                    . Highly enantioenriched acyloin products were obtained in good to high yields and several reactions were performed on a preparative scale. The newly introduced functionalized \u03b1\u2010keto acids, accessible by known aldolase\u2010catalyzed transformations, substantially broaden the donor substrate range of ThDP\u2010dependent enzymes, thus enabling a more general use of these already valuable catalysts.\n                  <\/jats:p>","DOI":"10.1002\/anie.202113405","type":"journal-article","created":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T01:51:17Z","timestamp":1643421077000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Unifying Scheme for the Biosynthesis of Acyl\u2010Branched Sugars: Extended Substrate Scope of Thiamine\u2010Dependent Enzymes"],"prefix":"10.1002","volume":"61","author":[{"given":"Jan\u2010Patrick","family":"Steitz","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Pharmazeutische Wissenschaften Albert-Ludwigs-Universit\u00e4t Freiburg  Albertstrasse 25 79104 Freiburg 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