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Here, we report a simple electrothermal flow\u2010enhanced biosensor for ultrafast, high sensitivity measurements of protein biomarkers in whole blood. Magnetic nanobeads dually\u2010labeled with a detection antibody and enzyme reporter are used to form immunocomplexes with the target protein, which are readily transported to the sensor via magnetic concentration. The incorporation of electrothermal flows enhances immunocomplex formation, allowing for rapid and sensitive detection without requiring blood purification or lengthy incubation. Proof of concept was carried out using <jats:italic>Plasmodium falciparum<\/jats:italic> histidine\u2010rich protein 2 (<jats:italic>Pf<\/jats:italic>HRP2), a malaria parasite biomarker, which could be detected at concentrations as low as 5.7\u2005pg\u2009mL<jats:sup>\u22121<\/jats:sup> (95\u2005fM) in whole blood in 7\u2005min. The speed, sensitivity and simplicity of this device make it attractive for rapid diagnostic testing.<\/jats:p>","DOI":"10.1002\/anie.202200206","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T06:31:36Z","timestamp":1647412296000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Ultrafast Electrothermal Flow\u2010Enhanced Magneto Biosensor for Highly Sensitive Protein Detection in Whole Blood"],"prefix":"10.1002","volume":"61","author":[{"given":"Jiran","family":"Li","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering Rice University  Houston TX 77005 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0289-2634","authenticated-orcid":false,"given":"Peter B.","family":"Lillehoj","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Rice University  Houston TX 77005 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