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The NMR\u2010based distinction between the signals of those sialic acids in the glycans covalently attached to the spike protein and those belonging to the exogenous \u03b12,3 and \u03b12,6 sialyl <jats:italic>N<\/jats:italic>\u2010acetyllactosamine ligands has been achieved by synthesizing uniformly <jats:sup>13<\/jats:sup>C\u2010labelled trisaccharides at the sialic acid and galactose moieties. STD\u2010<jats:sup>1<\/jats:sup>H,<jats:sup>13<\/jats:sup>C\u2010HSQC NMR experiments elegantly demonstrate the direct interaction of the sialic acid residues of both trisaccharides with additional participation of the galactose moieties, especially for the \u03b12,3\u2010linked analogue. Additional experiments with the spike protein in the presence of a specific antibody for the N\u2010terminal domain and with the isolated receptor binding and N\u2010terminal domains of the spike protein unambiguously show that the sialic acid binding site is located at the N\u2010terminal domain.<\/jats:p>","DOI":"10.1002\/anie.202201432","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T12:25:06Z","timestamp":1645532706000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["The SARS\u2010CoV\u20102 Spike Glycoprotein Directly Binds Exogeneous Sialic Acids: A NMR View"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8724-8783","authenticated-orcid":false,"given":"Luca","family":"Unione","sequence":"first","affiliation":[{"name":"CICbioGUNE Basque Research &amp; Technology Alliance (BRTA)  Bizkaia Technology Park, Building 800 48162 Derio Bizkaia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5120-2460","authenticated-orcid":false,"given":"Mar\u00eda J.","family":"Moure","sequence":"additional","affiliation":[{"name":"CICbioGUNE Basque Research &amp; 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