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Current sensitive chemiluminophores have issues of short half\u2010lives and low brightness, limiting their ability for in vivo longitudinal monitoring of immunological processes. To tackle these issues, we report benzoazole\u2010phenoxyl\u2010dioxetane (BAPD)\u2010based chemiluminophores with intramolecular hydrogen bonding for in vivo imaging of neutrophils. Compared with the classical counterpart, chemiluminescence half\u2010lives and brightness of BAPDs in the aqueous solution are increased by \u223c 33\u2010 and 8.2\u2010fold, respectively. Based on the BAPD scaffold, a neutrophil elastase\u2010responsive chemiluminescent probe is developed for real\u2010time imaging of neutrophils in peritonitis and psoriasis mouse models. 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