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A lidocaine\u2010based DES with the fatty acid oleic acid as a hydrogen\u2010bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026\u2009\u00b0C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (&gt;95\u2009%), whereas the hydrophobic substrate and educt accumulated (&gt;95\u2009%) in the DES\u2010enriched layer. Finally, a reduction catalyzed by horse liver alcohol dehydrogenase was performed in a larger\u2010scale experiment, and the biocatalyst could be recycled by simply removing the DES phase for three recycling runs.<\/jats:p>","DOI":"10.1002\/anie.202203823","type":"journal-article","created":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T08:19:39Z","timestamp":1652948379000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2591-0118","authenticated-orcid":false,"given":"Lars\u2010Erik","family":"Meyer","sequence":"first","affiliation":[{"name":"Department of Biological and Chemical Engineering Biocatalysis and Bioprocessing Group Aarhus University  Gustav Wieds Vej 10 8000 Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads Bruno","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering Biocatalysis and Bioprocessing Group Aarhus University  Gustav Wieds Vej 10 8000 Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6754-2814","authenticated-orcid":false,"given":"Selin","family":"Kara","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering Biocatalysis and Bioprocessing Group Aarhus University  Gustav Wieds Vej 10 8000 Aarhus Denmark"},{"name":"Institute of Technical Chemistry Leibniz University Hannover  Callinstr. 5 30167 Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,21]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"\u00a0"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4GC01149J"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40508-016-0051-z"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0gc00918k"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/b418069k"},{"key":"e_1_2_7_6_1","unstructured":"\u00a0"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1039\/D0OB02214D"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-018-0419-2"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/op0255231"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/sc5004314"},{"key":"e_1_2_7_11_1","unstructured":"\u00a0"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1039\/D0RE00458H"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/9783527818488"},{"key":"e_1_2_7_14_1","unstructured":"\u00a0"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-021-00044-z"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cogsc.2021.100514"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.1c00273"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.1c01742"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.0c01496"},{"key":"e_1_2_7_20_2","first-page":"113","volume":"60","author":"Wohlgemuth R.","year":"2021","journal-title":"Nat. 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