{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:33:53Z","timestamp":1789385633005,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"40","license":[{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2022,10,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ubiquitin (Ub)\u2010like protein ISG15 (interferon\u2010stimulated gene 15) regulates innate immunity and links with the evasion of host response by viruses such as SARS\u2010CoV\u20102. Dissecting ISGylation pathways recently received increasing attention which can inform related disease interventions, but such studies necessitate the preparation and development of various ISG15 protein tools. Here, we find that the leader protease (Lb<jats:sup>pro<\/jats:sup>) encoded by foot\u2010and\u2010mouth disease virus can promote ligation reactions between recombinant ISG15 and synthetic glycyl compounds, generating protein tools such as ISG15\u2010propargylamide and ISG15\u2010rhodamine110, which are needed for cellular proteomic studies of deISGylases, and the screening and evaluation of inhibitors against SARS\u2010CoV\u20102 papain\u2010like protease (PLpro). Furthermore, this strategy can be also used to load ISG15 onto the lysine of a synthetic peptide through an isopeptide bond, and prepare Ub and NEDD8 (ubiquitin\u2010like protein Nedd8) protein tools.<\/jats:p>","DOI":"10.1002\/anie.202206205","type":"journal-article","created":{"date-parts":[[2022,8,13]],"date-time":"2022-08-13T03:33:41Z","timestamp":1660361621000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Expedient Synthesis of Ubiquitin\u2010like Protein ISG15 Tools through Chemo\u2010Enzymatic Ligation Catalyzed by a Viral Protease Lb<sup>pro<\/sup>"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4007-7710","authenticated-orcid":false,"given":"Tian","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuntong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meijing","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiachen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lujun","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guochao","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Tian","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bioinformatics School of Life Sciences Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiteng","family":"Deng","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bioinformatics School of Life Sciences Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"He","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6290-8602","authenticated-orcid":false,"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Tsinghua University  Beijing 100084 P. R. China"},{"name":"Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at Microscale University of Science and Technology of China  Hefei 230026 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0750-7352","authenticated-orcid":false,"given":"Jinghong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Tsinghua University  Beijing 100084 P. R. China"},{"name":"Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at Microscale University of Science and Technology of China  Hefei 230026 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,29]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3787"},{"key":"e_1_2_7_2_1","unstructured":"\u00a0"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-093010-153308"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.6b00737"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0020-5"},{"key":"e_1_2_7_6_1","unstructured":"\u00a0"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2601-5"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-01035-8"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00884-1"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2019.00923"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3371"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.1c00519"},{"key":"e_1_2_7_13_1","unstructured":"\u00a0"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.1.84-95.2004"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(05)99003-4"},{"key":"e_1_2_7_16_1","unstructured":"\u00a0"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.1c01490"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-020-0528-y"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201300608"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja300500a"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-019-0227-4"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26812-9"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1710617115"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1482-y"},{"key":"e_1_2_7_25_1","unstructured":"\u00a0"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2013.02.039"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja309802n"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1013388108"},{"key":"e_1_2_7_29_1","unstructured":"\u00a0"},{"key":"e_1_2_7_30_2","doi-asserted-by":"crossref","unstructured":"C.\u2005M. Loch C.\u2005L. Cuccherini C.\u2005A. Leach J.\u2005E. Strickler Proteomics2011 10 M110 002402;","DOI":"10.1074\/mcp.M110.002402"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-1100"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2019.104661"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2272-7_12"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109078200"},{"key":"e_1_2_7_35_1","unstructured":"\u00a0"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000679"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2011.17"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.05.002"},{"key":"e_1_2_7_39_1","unstructured":"\u00a0"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2007.07.034"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020106275"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2021.05.012"},{"key":"e_1_2_7_43_1","unstructured":"\u00a0"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.81"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2015.37"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.152"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00630-8"},{"key":"e_1_2_7_48_1","unstructured":"\u00a0"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2021.04.020"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20210244"},{"key":"e_1_2_7_51_1","unstructured":"\u00a0"},{"key":"e_1_2_7_52_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-021-00836-9"},{"key":"e_1_2_7_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20718-8"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-021-00570-0"},{"key":"e_1_2_7_55_1","unstructured":"\u00a0"},{"key":"e_1_2_7_56_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00450"},{"key":"e_1_2_7_57_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.8b00712"},{"key":"e_1_2_7_58_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201203843"},{"key":"e_1_2_7_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201100996"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2018.05.081"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202206205","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.202206205","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202206205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T08:02:28Z","timestamp":1692604948000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.202206205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,29]]},"references-count":60,"journal-issue":{"issue":"40","published-print":{"date-parts":[[2022,10,4]]}},"alternative-id":["10.1002\/anie.202206205"],"URL":"https:\/\/doi.org\/10.1002\/anie.202206205","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,29]]},"assertion":[{"value":"2022-04-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202206205"}}