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Herein, we report a novel one\u2010pot multi\u2010segments ligation strategy using N\u2010terminal thiazolidine (Thz) peptide and a newly designed formaldehyde scavenger. Among the designed 2\u2010aminobenzamide\u2010based aldehyde scavengers, 2\u2010amino\u20105\u2010methoxy\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2032,\n                    <jats:italic>N<\/jats:italic>\n                    \u2032\u2010dimethylbenzohydrazide (AMDBH) can remarkably convert Thz into unprotected cysteine at pH\u20054.0. Furthermore, AMDBH degrades Thz at a considerably low rate at pH\u20057.5, and thioester degradation caused by this scavenger is negligible. As a result, we have developed an efficient one\u2010pot peptide ligation strategy by simply repetitively changing the pH with AMDBH. Finally, we synthesize mono\u2010ubiquitinated histone H2A.Z (209 amino acids) via AMDBH\u2010mediated one\u2010pot four\u2010segment peptide ligation in good yield.\n                  <\/jats:p>","DOI":"10.1002\/anie.202206240","type":"journal-article","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T16:30:52Z","timestamp":1658853052000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Repetitive Thiazolidine Deprotection Using a Thioester\u2010Compatible Aldehyde Scavenger for One\u2010Pot Multiple Peptide Ligation**"],"prefix":"10.1002","volume":"61","author":[{"given":"Koki","family":"Nakatsu","sequence":"first","affiliation":[{"name":"Department of Biomolecular Engineering Graduate School of Engineering Nagoya University  Furo-cho Chikusa-ku Nagoya 464-8603 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