{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:46:46Z","timestamp":1792028806013,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"39","license":[{"start":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T00:00:00Z","timestamp":1661385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004325","name":"AstraZeneca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004325","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2022,9,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010driven enzymes are of great interest for industrial biotransformations. Herein, we show for the first time that oxalate oxidase (OXO) is an efficient in situ source of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    for one of these biocatalysts, which is known as unspecific peroxygenase (UPO). OXO is reasonably robust, produces only CO\n                    <jats:sub>2<\/jats:sub>\n                    as a by\u2010product and uses oxalate as a cheap sacrificial electron donor. UPO has significant potential as an industrial catalyst for selective C\u2212H oxyfunctionalisations, as we confirm herein by testing a diverse drug panel using miniaturised high\u2010throughput assays and mass spectrometry. 33 out of 64 drugs were converted in 5\u2005\u03bcL\u2010scale reactions by the UPO with OXO (conversion &gt;70\u2009% for 11 drugs). Furthermore, oxidation of the drug tolmetin was achieved on a 50\u2005mg scale (TON\n                    <jats:sub>UPO<\/jats:sub>\n                    25\u2009664) with 84\u2009% yield, which was further improved via enzyme immobilization. This one\u2010pot approach ensures adequate H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    levels, enabling rapid access to industrially relevant molecules that are difficult to obtain by other routes.\n                  <\/jats:p>","DOI":"10.1002\/anie.202207831","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T15:40:38Z","timestamp":1659454838000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Oxalate Oxidase for In Situ H\n                    <sub>2<\/sub>\n                    O\n                    <sub>2<\/sub>\n                    \u2010Generation in Unspecific Peroxygenase\u2010Catalysed Drug Oxyfunctionalisations**"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4199-9458","authenticated-orcid":false,"given":"Elvira","family":"Romero","sequence":"first","affiliation":[{"name":"Compound Synthesis and Management Discovery Sciences BioPharmaceuticals R&amp;D AstraZeneca  Pepparedsleden 1 43183 M\u00f6lndal Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0904-2835","authenticated-orcid":false,"given":"Magnus J.","family":"Johansson","sequence":"additional","affiliation":[{"name":"Medicinal Chemistry Research and Early Development Cardiovascular, Renal and Metabolism (CVRM) BioPharmaceuticals R&amp;D AstraZeneca  Pepparedsleden 1 43183 M\u00f6lndal Sweden"},{"name":"Department of Organic Chemistry Stockholm University  Svante Arrhenius v\u00e4g 16\u2005C 10691 Stockholm 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