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It contains an anthraquinone moiety and an unusual halogenated xanthone moiety connected by a bicyclo[3.2.2]nonane. During elucidation of the biosynthetic pathway of beticolin 1, a novel non\u2010heme iron oxygenase BTG13 responsible for anthraquinone ring cleavage was discovered. More importantly, the discovery of non\u2010heme iron oxygenase BTG13 is well supported by experimental evidence: (i)\u2005crystal structure and the inductively coupled plasma mass spectrometry revealed that its reactive site is built by an atypical iron ion coordination, where the iron ion is uncommonly coordinated by four histidine residues, an unusual carboxylated\u2010lysine (Kcx377) and water; (ii)\u2005Kcx377 is mediated by His58 and Thr299 to modulate the catalytic activity of BTG13. Therefore, we believed this study updates our knowledge of metalloenzymes.<\/jats:p>","DOI":"10.1002\/anie.202208772","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T15:37:42Z","timestamp":1658417862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Discovery of the Biosynthetic Pathway of Beticolin 1 Reveals a Novel Non\u2010Heme Iron\u2010Dependent Oxygenase for Anthraquinone Ring Cleavage"],"prefix":"10.1002","volume":"61","author":[{"given":"Xiaodong","family":"Hou","sequence":"first","affiliation":[{"name":"Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education School of Biotechnology Jiangnan University  Wuxi 214122 P. R. 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