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Here, we develop a ready\u2010to\u2010use and multimodal detection based on a rebuilding\u2010free, ultrasensitive and selective strategy named dual hairpin ligation\u2010induced isothermal amplification pro (DHLApro). Taking influenza A, influenza B, MERS\u2010CoV, SARS\u2010CoV\u20102 as model targets, we demonstrate DHLApro provides \u2248zM level ultra\u2010sensitivity, being equaling to 0.45\u2005copy\/\u03bcL in original sample. By simply changing the recognition module, a set of DHLApro components can be applied to a new target without performance loss. Moreover, DHLApro innovatively allows flexible logic\/multiplex assay using one set of primer, for example, the \u201cN pathogens\u2010in\u20101\u201d OR gate screening and accurate multi\u2010channel multiplex assay. Compared with traditional methods, the cost of this logic\/multiplex assay has been largely reduced and the cross\u2010interference between the multiple primer sets is also avoided.<\/jats:p>","DOI":"10.1002\/anie.202209496","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T13:23:15Z","timestamp":1659964995000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Rebuilding\u2010Free Nucleic Acid Detection Strategy Enables Ultrasensitive Genotyping, N\u2010in\u20101 Logic Screening and Accurate Multiplex Assay of Dangerous Pathogens"],"prefix":"10.1002","volume":"61","author":[{"given":"Huan","family":"Li","sequence":"first","affiliation":[{"name":"State Key Lab of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin, 130022 China"},{"name":"University of Science and Technology of China Hefei Anhui 230026 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiying","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Northeast Normal University Changchun Jilin, 130024 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yidan","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Lab of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin, 130022 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaning","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Lab of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin, 130022 China"},{"name":"University of Science and Technology of China Hefei Anhui 230026 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Lab of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin, 130022 China"},{"name":"University of Science and Technology of China Hefei Anhui 230026 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7224-0041","authenticated-orcid":false,"given":"Bingling","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Lab of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin, 130022 China"},{"name":"University of Science and Technology of China Hefei Anhui 230026 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,25]]},"reference":[{"key":"e_1_2_3_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040204"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00246"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-27076-z"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c03456"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2013.07.017"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201503182"},{"key":"e_1_2_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201503182"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c03918"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201606060"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201606060"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202014506"},{"key":"e_1_2_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202014506"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c03702"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-020-00617-5"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c00007"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c01829"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202110819"},{"key":"e_1_2_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202110819"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abh2848"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202115907"},{"key":"e_1_2_3_17_2","doi-asserted-by":"crossref","first-page":"e202115907","DOI":"10.1002\/ange.202115907","volume":"134","year":"2022","journal-title":"Angew. 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