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Detection of lactate has been relying on a few enzymes that need additional oxidants. In this work, DNA aptamers for L\u2010lactate were obtained using a library\u2010immobilization selection method and the highest affinity aptamer reached a\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d<\/jats:sub>\n                    of 0.43\u2005mM as determined using isothermal titration calorimetry. The aptamers showed up to 50\u2010fold selectivity for L\u2010lactate over D\u2010lactate and had little responses to other closely related analogs such as pyruvate or 3\u2010hydroxybutyrate. A fluorescent biosensor based on the strand displacement method showed a limit of detection of 0.55\u2005mM L\u2010lactate, and the sensor worked in 90\u2009% serum. Simultaneous detection of L\u2010lactate and D\u2010glucose in the same solution was achieved. This work has broadened the scope of aptamers to simple metabolites and provided a useful probe for continuous and multiplexed monitoring.\n                  <\/jats:p>","DOI":"10.1002\/anie.202212879","type":"journal-article","created":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T22:04:07Z","timestamp":1674597847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["Simultaneous Detection of L\u2010Lactate and D\u2010Glucose Using DNA Aptamers in Human Blood Serum**"],"prefix":"10.1002","volume":"62","author":[{"given":"Po\u2010Jung Jimmy","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Chemistry Waterloo Institute for Nanotechnology University of Waterloo  200 University Avenue West Waterloo Ontario N2L 3G1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5918-9336","authenticated-orcid":false,"given":"Juewen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Waterloo Institute for Nanotechnology University of Waterloo  200 University Avenue West Waterloo Ontario N2L 3G1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,9]]},"reference":[{"key":"e_1_2_3_1_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3419034"},{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2016.07.076"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000000405"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2018.11.004"},{"key":"e_1_2_3_5_1","first-page":"71","volume":"78","author":"Przybyt M.","year":"2014","journal-title":"Biotechnol. 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