{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:30:13Z","timestamp":1786977013164,"version":"build-2736575974"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,12,9]],"date-time":"2023-12-09T00:00:00Z","timestamp":1702080000000},"content-version":"am","delay-in-days":345,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T00:00:00Z","timestamp":1672272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073714"],"award-info":[{"award-number":["82073714"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22007102"],"award-info":[{"award-number":["22007102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["Hatch project ARZT-1361640-H12-224"],"award-info":[{"award-number":["Hatch project ARZT-1361640-H12-224"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007167","name":"Teknologian Tutkimuskeskus VTT","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007167","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Orsellinic acid (OA) derivatives are produced by filamentous fungi using nonreducing polyketide synthases (nrPKSs). The chain\u2010releasing thioesterase (TE) domains of such nrPKSs were proposed to also catalyze dimerization to yield didepsides, such as lecanoric acid. Here, we use combinatorial domain exchanges, domain dissections and reconstitutions to reveal that the TE domain of the lecanoric acid synthase Preu6 of\n                    <jats:italic>Preussia isomera<\/jats:italic>\n                    must collaborate with the starter acyl transferase (SAT) domain from the same nrPKS. We show that artificial SAT\u2010TE fusion proteins are highly effective catalysts and reprogram the ketide homologation chassis to form didepsides. We also demonstrate that dissected SAT and TE domains of Preu6 physically interact, and SAT and TE domains of OA\u2010synthesizing nrPKSs may co\u2010evolve. Our work highlights an unexpected domain\u2013domain interaction in nrPKSs that must be considered for the combinatorial biosynthesis of unnatural didepsides, depsidones, and diphenyl ethers.\n                  <\/jats:p>","DOI":"10.1002\/anie.202214379","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T10:46:07Z","timestamp":1670582767000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Didepside Formation by the Nonreducing Polyketide Synthase Preu6 of\n                    <i>Preussia isomera<\/i>\n                    Requires Interaction of Starter Acyl Transferase and Thioesterase Domains"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1424-0991","authenticated-orcid":false,"given":"Qingpei","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaibiao","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianhua","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifu","family":"Gong","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yigui","family":"Mao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry Guangxi Academy of Sciences  No.98 Daling Road Nanning 530007 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengfeng","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry Guangxi Academy of Sciences  No.98 Daling Road Nanning 530007 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3627-0454","authenticated-orcid":false,"given":"Istv\u00e1n","family":"Moln\u00e1r","sequence":"additional","affiliation":[{"name":"VTT Technical Research Centre of Finland, Division of Industrial Biotechnology and Food Solutions  Tietotie 2 Espoo 02150 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South-Central Minzu University  182 Minyuan Road Hongshan District Wuhan 430074 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,29]]},"reference":[{"key":"e_1_2_3_1_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CC00855A"},{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo11100683"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/md16110451"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2330"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja071038f"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201300626"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.9b00768"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c02813"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.27.587"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.56.1551"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00300-009-0602-9"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.819086"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2020.103481"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0cc01162b"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja4041362"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1301201110"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406999111"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.6b00110"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.0c07050"},{"key":"e_1_2_3_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8OB02773K"},{"key":"e_1_2_3_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27144473"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202214379","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.202214379","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/anie.202214379","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202214379","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T07:30:07Z","timestamp":1692257407000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.202214379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,29]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1002\/anie.202214379"],"URL":"https:\/\/doi.org\/10.1002\/anie.202214379","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,29]]},"assertion":[{"value":"2022-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202214379"}}