{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:48:07Z","timestamp":1788994087266,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T00:00:00Z","timestamp":1680480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30-CA14051"],"award-info":[{"award-number":["P30-CA14051"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2023,5,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b1\u2010Klotho, an aging\u2010related protein found in the kidney, parathyroid gland, and choroid plexus, acts as an essential co\u2010receptor with the fibroblast growth factor 23 receptor complex to regulate serum phosphate and vitamin D levels. Decreased levels of \u03b1\u2010Klotho are a hallmark of age\u2010associated diseases. Detecting or labeling \u03b1\u2010Klotho in biological milieu has long been a challenge, however, hampering the understanding of its role. Here, we developed branched peptides by single\u2010shot parallel automated fast\u2010flow synthesis that recognize \u03b1\u2010Klotho with improved affinity relative to their monomeric versions. These peptides were further shown to selectively label Klotho for live imaging in kidney cells. Our results demonstrate that automated flow technology enables rapid synthesis of complex peptide architectures, showing promise for future detection of \u03b1\u2010Klotho in physiological settings.<\/jats:p>","DOI":"10.1002\/anie.202300289","type":"journal-article","created":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T04:00:07Z","timestamp":1678420807000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Branched Multimeric Peptides as Affinity Reagents for the Detection of \u03b1\u2010Klotho Protein"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5342-9244","authenticated-orcid":false,"given":"Xiyun","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Chemistry Massachusetts Institute of Technology  77 Massachusetts Avenue 02139 Cambridge MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4086-3436","authenticated-orcid":false,"given":"Peiyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry Massachusetts Institute of Technology  77 Massachusetts Avenue 02139 Cambridge MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C. K.","family":"Wang","sequence":"additional","affiliation":[{"name":"Calico Life Sciences  1170 Veterans Boulevard 94080 South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey L.","family":"Smith","sequence":"additional","affiliation":[{"name":"AbbVie Bioresearch Center  100 Research Drive 01605 Worcester MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvino","family":"Sousa","sequence":"additional","affiliation":[{"name":"AbbVie Bioresearch Center  100 Research Drive 01605 Worcester MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5640-1645","authenticated-orcid":false,"given":"Andrei","family":"Loas","sequence":"additional","affiliation":[{"name":"Department of Chemistry Massachusetts Institute of Technology  77 Massachusetts Avenue 02139 Cambridge MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan 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