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The current study shows that directed evolution can be used to engineer variants of the dioxygenase SadX that address this challenge. Site\u2010selective azidation of succinylated amino acids and a succinylated amine was achieved as a result of mutations throughout the SadX structure. The installed azide group was reduced to a primary amine, and the succinyl group required for azidation was enzymatically cleaved to provide the corresponding amine. These results provide a promising starting point for evolving additional SadX variants with activity on structurally distinct substrates and for enabling enzymatic C\u2212H functionalization with other non\u2010native functional groups.\n                  <\/jats:p>","DOI":"10.1002\/anie.202301370","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T11:53:57Z","timestamp":1675943637000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Directed Evolution of an Iron(II)\u2010 and \u03b1\u2010Ketoglutarate\u2010Dependent Dioxygenase for Site\u2010Selective Azidation of Unactivated Aliphatic C\u2212H Bonds**"],"prefix":"10.1002","volume":"62","author":[{"given":"Christian A.","family":"Gomez","sequence":"first","affiliation":[{"name":"Department of Chemistry Indiana University  Bloomington IN 47405 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dibyendu","family":"Mondal","sequence":"additional","affiliation":[{"name":"Department of Chemistry Indiana University  Bloomington IN 47405 USA"},{"name":"Kalsec Inc.  3713W. 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