{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T21:38:53Z","timestamp":1789681133301,"version":"4.0.1"},"reference-count":42,"publisher":"Wiley","issue":"35","license":[{"start":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T00:00:00Z","timestamp":1689552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2023,8,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nuclear Magnetic Resonance (NMR) spectra of human serum and plasma show, besides metabolites and lipoproteins, two characteristic signals termed GlycA and B arising from the acetyl groups of glycoprotein glycans from acute phase proteins, which constitute good markers for inflammatory processes. Here, we report a comprehensive assignment of glycoprotein glycan NMR signals observed in human serum, showing that GlycA and GlycB signals originate from Neu5Ac and GlcNAc moieties from <jats:italic>N\u2010<\/jats:italic>glycans, respectively. Diffusion\u2010edited NMR experiments demonstrate that signal components can be associated with specific acute phase proteins. Conventionally determined concentrations of acute phase glycoproteins correlate well with distinct features in NMR spectra (<jats:italic>R<\/jats:italic><jats:sup><jats:italic>2<\/jats:italic><\/jats:sup> up to 0.9422, <jats:italic>p<\/jats:italic>\u2010value &lt;0.001), allowing the simultaneous quantification of several acute phase inflammation proteins. Overall, a proteo\u2010metabolomics NMR signature of significant diagnostic potential is obtained within 10\u201320\u2005min acquisition time. This is exemplified in serum samples from COVID\u201019 and cardiogenic shock patients showing significant changes in several acute phase proteins compared to healthy controls.<\/jats:p>","DOI":"10.1002\/anie.202306154","type":"journal-article","created":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T14:27:55Z","timestamp":1687357675000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Towards a Precise NMR Quantification of Acute Phase Inflammation Proteins from Human Serum"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5825-4407","authenticated-orcid":false,"given":"Alvaro","family":"Mallagaray","sequence":"first","affiliation":[{"name":"Institute of Chemistry and Metabolomics University of L\u00fcbeck  Ratzeburger Allee 160 23562 L\u00fcbeck 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L\u00fcbeck and Medical Center Schleswig-Holstein Campus L\u00fcbeck  Ratzeburger Allee 160 23538 L\u00fcbeck Germany"},{"name":"Fraunhofer Research Institution for Individualized and Cell-Based Medical Engineering (IMTE)  M\u00f6nkhofer Weg 239 a 23538 L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Petersmann","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine Greifswald University Hospital  Fleischmannstra\u00dfe 8 17475 Greifswald Germany"},{"name":"Institute of Clinical Chemistry and Laboratory Medicine Carl von Ossietzky University  Ammerl\u00e4nder Heerstra\u00dfe 114\u2013118 26129 Oldenburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6678-7964","authenticated-orcid":false,"given":"Matthias","family":"Nauck","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine 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