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The RCD has nanomolar affinity for EPX, shows high selectivity against related peroxidases and other eosinophil proteins, and is resistant to degradation by mammalian nucleases. An optimized RCD was used to develop both fluorescence and lateral flow assays, which were evaluated using 38\u2005minimally processed patient sputum samples (23\u2005non\u2010eosinophilic, 15\u2005eosinophilic), producing a clinical sensitivity of 100\u2009% and specificity of 96\u2009%. 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