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To identify suitable MTs, we developed a continuous fluorescence\u2010based, high\u2010throughput assay for SAM\u2010dependent methyltransferases, which facilitates screening using\n                    <jats:italic>E. coli<\/jats:italic>\n                    cell lysates. This assay involves two enzymatic steps for the conversion of\n                    <jats:italic>S<\/jats:italic>\n                    \u2010adenosyl\u2010\n                    <jats:sc>l<\/jats:sc>\n                    \u2010homocysteine into H\n                    <jats:sub>2<\/jats:sub>\n                    S to result in a selective fluorescence readout via reduction of an azidocoumarin sulfide probe. Investigation of two\n                    <jats:italic>O<\/jats:italic>\n                    \u2010MTs and an\n                    <jats:italic>N<\/jats:italic>\n                    \u2010MT confirmed that this assay is suitable for the determination of methyltransferase activity in\n                    <jats:italic>E. coli<\/jats:italic>\n                    cell lysates.\n                  <\/jats:p>","DOI":"10.1002\/anie.202313912","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T17:25:05Z","timestamp":1698945905000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A Universal, Continuous Assay for SAM\u2010dependent Methyltransferases"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5887-3045","authenticated-orcid":false,"given":"Marian J.","family":"Menke","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Enzyme Catalysis Institute of Biochemistry University of Greifswald  Felix-Hausdorff-Str. 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Schneider","sequence":"additional","affiliation":[{"name":"Discovery Sciences BioPharmaceuticals R&amp;D AstraZeneca  Pepparedsleden 1 43183 M\u00f6lndal Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5874-4577","authenticated-orcid":false,"given":"Christoffel P. 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