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Using the enzymatic catalysis of ALP that continuously catalyses the dephosphorylation and self\u2010assembly of trans\u2010cyclooctene (TCO)\u2010bearing\n                    <jats:bold>P\u2010FFGd\u2010TCO<\/jats:bold>\n                    , a high density of fluorescent and magnetic TCO\u2010containing nanoparticles (\n                    <jats:bold>FMNPs\u2010TCO<\/jats:bold>\n                    ) can be synthesized and retained on the membrane of tumor cells. They can act as \u2018artificial antigens\u2019 amenable to concurrently capture lately administrated tetrazine (Tz)\u2010decorated PS (\n                    <jats:bold>775NP\u2010Tz<\/jats:bold>\n                    ) and carbonic anhydrase (CA) inhibitor (\n                    <jats:bold>SA\u2010Tz<\/jats:bold>\n                    ) via the fast IEDDA reaction. This two\u2010step pretargeting process can further induce\n                    <jats:bold>FMNPs\u2010TCO<\/jats:bold>\n                    regrowth into microparticles (\n                    <jats:bold>FMNPs\u2010775\/SA<\/jats:bold>\n                    ) directly on tumor cell membranes, which is analyzed by bio\u2010SEM and fluorescence imaging. Thus, efficient enrichment of both\n                    <jats:bold>SA\u2010Tz<\/jats:bold>\n                    and\n                    <jats:bold>775NP\u2010Tz<\/jats:bold>\n                    in tumors can be achieved, allowing to alleviate hypoxia by continuously inhibiting CA activity and improving PDT of tumors. Findings show that subcutaneous HeLa tumors could be completely eradicated and no tumor recurred after irradiation with an 808\u2005nm laser (0.33\u2005W\u2009cm\n                    <jats:sup>\u22122<\/jats:sup>\n                    , 10\u2005min). This pretargeted approach may be applied to enrich other therapeutic agents in tumors to improve targeted therapy.\n                  <\/jats:p>","DOI":"10.1002\/anie.202314039","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T20:32:45Z","timestamp":1701894765000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Cascade In Situ Self\u2010Assembly and Bioorthogonal Reaction Enable the Enrichment of Photosensitizers and Carbonic Anhydrase Inhibitors for Pretargeted Cancer Theranostics"],"prefix":"10.1002","volume":"63","author":[{"given":"Xidan","family":"Wen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University  Nanjing 210023 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Nanjing University  Nanjing 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010Juan","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University  Nanjing 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9887-0914","authenticated-orcid":false,"given":"Deju","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University  Nanjing 210023 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