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However, classical organic fluorophore motifs feature large overlap between excitation and emission spectra, leading to the requirement of advanced optical set up to filter desired signal, which limits their application in scenarios with simple settings. Here, a series of wavelength\u2010tunable small\u2010molecule fluorescent dyes (<jats:bold>PTs<\/jats:bold>) bearing simple organic moieties have been developed, which exhibit Stokes shift up to 262\u2005nm, molar extinction coefficients ranged 30,000\u2013100,000\u2005M<jats:sup>\u22121<\/jats:sup>\u2009cm<jats:sup>\u22121<\/jats:sup>, with quantum yields up to 54.8\u2009%. Furthermore, these dyes were formulated into fluorescent nanoparticles (<jats:bold>PT\u2010NPs<\/jats:bold>), and applied in lateral flow assay (LFA). Consequently, limit of detection for SARS\u2010CoV\u20102 nucleocapsid protein reached 20\u2005fM with naked eye, a 100\u2010fold improvement in sensitivity compared to the pM detection level for colloidal gold\u2010based LFA. Besides, combined with loop\u2010mediated isothermal amplification (LAMP), the LFA system achieved the visualization of single copy level nucleic acid detection for monkeypox (Mpox).<\/jats:p>","DOI":"10.1002\/anie.202318800","type":"journal-article","created":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T03:03:39Z","timestamp":1709694219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Organic Fluorophores with Large Stokes Shift for the Visualization of Rapid Protein and Nucleic Acid Assays"],"prefix":"10.1002","volume":"63","author":[{"given":"Jingkai","family":"Yang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering Southern University of Science and Technology  1088 Xueyuan Road Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Xu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering Southern University of Science and Technology  1088 Xueyuan Road Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic and Nature Molecule Chemistry of Ministry of Education, Department of Chemistry &amp; Materials Science Northwest University. 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