{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:29:50Z","timestamp":1786976990456,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM61153"],"award-info":[{"award-number":["GM61153"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R35GM126961-01"],"award-info":[{"award-number":["1R35GM126961-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2024,5,27]]},"abstract":"Abstract<\/jats:title>\n \n The paper aims to elucidate the final stages in the biosynthesis of the [2Fe]\n H<\/jats:sub>\n active site of the [FeFe]\u2010hydrogenases. The recently hypothesized intermediate [Fe\n 2<\/jats:sub>\n (SCH\n 2<\/jats:sub>\n NH\n 2<\/jats:sub>\n )\n 2<\/jats:sub>\n (CN)\n 2<\/jats:sub>\n (CO)\n 4<\/jats:sub>\n ]\n 2\u2212<\/jats:sup>\n ([\n 1<\/jats:bold>\n ]\n 2\u2212<\/jats:sup>\n ) was prepared by a multistep route from [Fe\n 2<\/jats:sub>\n (S\n 2<\/jats:sub>\n )(CN)(CO)\n 5<\/jats:sub>\n ]\n \u2212<\/jats:sup>\n . The following synthetic intermediates were characterized in order: [Fe\n 2<\/jats:sub>\n (SCH\n 2<\/jats:sub>\n NHFmoc)\n 2<\/jats:sub>\n (CNBEt\n 3<\/jats:sub>\n )(CO)\n 5<\/jats:sub>\n ]\n \u2212<\/jats:sup>\n , [Fe\n 2<\/jats:sub>\n (SCH\n 2<\/jats:sub>\n NHFmoc)\n 2<\/jats:sub>\n (CN)\u2212(CO)\n 5<\/jats:sub>\n ]\n \u2212<\/jats:sup>\n , and [Fe\n 2<\/jats:sub>\n (SCH\n 2<\/jats:sub>\n NHFmoc)\n 2<\/jats:sub>\n (CN)\n 2<\/jats:sub>\n (CO)\n 4<\/jats:sub>\n ]\n 2\u2212<\/jats:sup>\n , where Fmoc is fluorenylmethoxycarbonyl). Derivatives of these anions include [K(18\u2010crown\u20106)]\n +<\/jats:sup>\n , PPh\n 4<\/jats:sub>\n +<\/jats:sup>\n and PPN\n +<\/jats:sup>\n salts as well as the\n 13<\/jats:sup>\n CD\n 2<\/jats:sub>\n \u2010isotopologues. These Fe\n 2<\/jats:sub>\n species exist as a mixture of two isomers attributed to diequatorial (ee) and axial\u2010equatorial (ae) stereochemistry at sulfur. In vitro experiments demonstrate that [\n 1<\/jats:bold>\n ]\n 2\u2212<\/jats:sup>\n maturates HydA1 in the presence of HydF and a cocktail of reagents. HydA1 can also be maturated using a highly simplified cocktail, omitting HydF and other proteins. This result is consistent with HydA1 participating in the maturation process and refines the roles of HydF.\n <\/jats:p>","DOI":"10.1002\/anie.202404044","type":"journal-article","created":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T11:23:55Z","timestamp":1711711435000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Final Stages in the Biosynthesis of the [FeFe]\u2010Hydrogenase Active Site"],"prefix":"10.1002","volume":"63","author":[{"given":"Xin","family":"Yu","sequence":"first","affiliation":[{"name":"School of Chemical Sciences University of Illinois at Urbana-Champaign Urbana IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guodong","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of California Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. David","family":"Britt","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of California Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2547-5128","authenticated-orcid":false,"given":"Thomas B.","family":"Rauchfuss","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences University of Illinois at Urbana-Champaign Urbana IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"key":"e_1_2_3_1_1","unstructured":"\u00a0"},{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2021.214191"},{"key":"e_1_2_3_3_2","first-page":"0000","author":"Sidabras J. W.","year":"2023","journal-title":"JBIC J. Biol. Inorg. 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