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The dye core displays large Stokes shift (in H<jats:sub>2<\/jats:sub>O, abs.\/emission max. 480\/550\u2005nm; \u03f5~19\u2009000, \u03c4<jats:sub>fl<\/jats:sub>&gt;4 ns), strong binding with the host (~560\u2005nM <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>) and a linker affording fluorescence detection of bioconjugates with antibody and nanobody. Combination of protein\u2010functionalized DPP dye with CB7 improves photostability and affords up to 12\u2010fold emission gain. Two\u2010color confocal and stimulated emission depletion (STED) microscopy with 595\u2005nm or 655\u2005nm STED depletion lasers shows that the presence of CB7 not only leads to improved brightness and image quality, but also results in DPP becoming cell\u2010permeable.<\/jats:p>","DOI":"10.1002\/anie.202410217","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T09:10:44Z","timestamp":1718615444000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Supramolecular Complex of Cucurbit[7]uril with Diketopyrrolopyrole Dye: Fluorescence Boost, Biolabeling and Optical Microscopy"],"prefix":"10.1002","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5647-1659","authenticated-orcid":false,"given":"Dojin","family":"Kim","sequence":"first","affiliation":[{"name":"Department of NanoBiophotonics Max Planck Institute for Multidisciplinary Sciences (MPI-NAT)  Am Fassberg 11 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