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NIR\u2010II) light enables deeper tissue penetration due to reduced tissue scattering as well as minimal background autofluorescence. SWIR\u2010emitting semiconductor quantum dots (QDs) with tunable emission peaks and optical stability are powerful contrast agents, yet few imaging demonstrations exclusively use SWIR emission beyond two\u2010color imaging schemes. In this study, we engineered three high quality lead sulfide\/cadmium sulfide (PbS\/CdS) core\/shell QDs with distinct SWIR emission peaks ranging from 1100\u20131550\u2005nm for simultaneous three\u2010color imaging in mice. We first use the exceptional photostability of QDs to non\u2010invasively track lymphatic drainage with longitudinal imaging, highlighting the detailed networks of lymphatic vessels with widefield imaging over a 2 hr period. We then perform multiplexed imaging with all three QDs to distinctly visualize the lymphatic system and spatially overlapping vasculature networks, including clearly distinguishing the liver and spleen. This work establishes optimized SWIR QDs for next generation multiplexed and longitudinal preclinical imaging, unlocking numerous opportunities for preclinical studies of disease progression, drug biodistribution, and cell trafficking dynamics in living organisms.<\/jats:p>","DOI":"10.1002\/anie.202410936","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T23:15:55Z","timestamp":1721171755000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Multiplexed Shortwave Infrared Imaging Highlights Anatomical Structures in Mice"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9133-0018","authenticated-orcid":false,"given":"Xingjian","family":"Zhong","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Northeastern University  Boston MA USA"},{"name":"Department of Biomedical Engineering Boston University  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3585-4431","authenticated-orcid":false,"given":"Amish","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Northeastern University  Boston MA USA"},{"name":"Department of Biomedical Engineering Boston University  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9147-5735","authenticated-orcid":false,"given":"Yidan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Northeastern University  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4225-0510","authenticated-orcid":false,"given":"Alexander M.","family":"Saeboe","sequence":"additional","affiliation":[{"name":"Division of Material Science &amp; Engineering Boston University  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5759-9905","authenticated-orcid":false,"given":"Allison M.","family":"Dennis","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Northeastern University  Boston MA USA"},{"name":"Department of Bioengineering Northeastern University  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,12]]},"reference":[{"key":"e_1_2_3_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.1c00600"},{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-016-0010"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/86684"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201705913"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05842-w"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.28.9.094805"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014501108"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5NH00073D"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201802394"},{"key":"e_1_2_3_10_1","first-page":"1043","volume":"6","author":"Thimsen E.","year":"2017","journal-title":"Nat. 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