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But easy folding of the target sequences causes remarkable deterioration of the recognition and shear efficiency in the case of single Cas\u2010CRISPR RNA (crRNA) duplex. Here, we develop a CRISPR\/Cas cooperative shearing (CRISPR\u2010CS) system. Compared with traditional CRISPR\/Cas system, two CRISPR\/Cas\u2010crRNA duplexes simultaneously recognize different sites in the target sequence, increasing recognition possibility and shearing efficiency. Cooperative shearing cuts more methylene blue\u2010ssDNA reporters on the electrode, enabling unamplified nucleic acid electrochemical assay in less than 5\u2005minutes with a detection limit of 9.5\u00d710\n                    <jats:sup>\u221220<\/jats:sup>\n                    \u2005M, 2 to 9 orders of magnitude lower than those of other electrochemical assays. The CRISPR\u2010CS platform detects monkeypox, human papilloma virus and amyotrophic lateral sclerosis with an accuracy up to 98.1\u2009%, demonstrating the potential application of the efficient cooperative shearing.\n                  <\/jats:p>","DOI":"10.1002\/anie.202411705","type":"journal-article","created":{"date-parts":[[2024,10,12]],"date-time":"2024-10-12T05:21:19Z","timestamp":1728710479000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["An Efficient CRISPR\/Cas Cooperative Shearing Platform for Clinical Diagnostics Applications"],"prefix":"10.1002","volume":"63","author":[{"given":"Junhong","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular Science Fudan University  Shanghai 200433 P. R. China"},{"name":"Laboratory of Molecular Materials and Devices Fudan University  Shanghai 200433 P. R. 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