{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T17:15:19Z","timestamp":1788628519038,"version":"build-2803163510"},"reference-count":63,"publisher":"Wiley","issue":"51","license":[{"start":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T00:00:00Z","timestamp":1733702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T00:00:00Z","timestamp":1733702400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Ja794\/11"],"award-info":[{"award-number":["Ja794\/11"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GRK 2039"],"award-info":[{"award-number":["GRK 2039"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009318","name":"Helmholtz Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009318","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2024,12,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Efficient labeling methods for protein visualization with minimal tag size and appropriate photophysical properties are required for single\u2010molecule localization microscopy (SMLM), providing insights into the organization and interactions of biomolecules in cells at the molecular level. Among the fluorescent light\u2010up aptamers (FLAPs) originally developed for RNA imaging, RhoBAST stands out due to its remarkable brightness, photostability, fluorogenicity, and rapid exchange kinetics, enabling super\u2010resolved imaging with high localization precision. Here, we expand the applicability of RhoBAST to protein imaging by fusing it to protein\u2010binding aptamers. The versatility of such bifunctional aptamers is demonstrated by employing a variety of protein\u2010binding aptamers and different FLAPs. Moreover, fusing RhoBAST with the GFP\u2010binding aptamer AP3 facilitates high\u2010 and super\u2010resolution imaging of GFP\u2010tagged proteins, which is particularly valuable in view of the widespread availability of plasmids and stable cell lines expressing proteins fused to GFP. The bifunctional aptamers compare favorably with standard antibody\u2010based immunofluorescence protocols, as they are 7\u2010fold smaller than antibody conjugates and exhibit higher bleaching\u2010resistance. We demonstrate the effectiveness of our approach in super\u2010resolution microscopy in secondary mammalian cell lines and primary neurons by RhoBAST\u2010PAINT, an SMLM protein imaging technique that leverages the transient binding of the fluorogenic rhodamine dye SpyRho to RhoBAST.<\/jats:p>","DOI":"10.1002\/anie.202412810","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T12:29:50Z","timestamp":1723120190000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Super\u2010Resolved Protein Imaging Using Bifunctional Light\u2010Up Aptamers"],"prefix":"10.1002","volume":"63","author":[{"given":"Franziska","family":"Gr\u00fcn","sequence":"first","affiliation":[{"name":"Institute of Pharmacy and Molecular Biotechnology (IPMB) Heidelberg University  69120 Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"van den Bergh","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Molecular Biotechnology (IPMB) Heidelberg University  69120 Heidelberg Germany"},{"name":"Department of Nuclear Medicine Heidelberg University Hospital  69120 Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maja","family":"Klevanski","sequence":"additional","affiliation":[{"name":"Department of Functional Neuroanatomy Heidelberg University  69120 Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mrigank S.","family":"Verma","sequence":"additional","affiliation":[{"name":"Institute of Applied Physics (APH) Karlsruhe Institute of Technology  76131 Karlsruhe Germany"},{"name":"Department of Applied Physics and Science Education Eindhoven University of Technology  5612AP Eindhoven Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bastian","family":"B\u00fchler","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology Max Planck Institute for Medical Research  69120 Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Ulrich","family":"Nienhaus","sequence":"additional","affiliation":[{"name":"Institute of Applied Physics (APH) Karlsruhe Institute of Technology  76131 Karlsruhe Germany"},{"name":"Institute of Nanotechnology (INT) Karlsruhe Institute of Technology  76344 Eggenstein-Leopoldshafen Germany"},{"name":"Institute of Biological and Chemical Systems (IBCS) Karlsruhe Institute of Technology  76344 Eggenstein-Leopoldshafen Germany"},{"name":"Department of Physics University of Illinois at Urbana-Champaign  Urbana IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kuner","sequence":"additional","affiliation":[{"name":"Department of Functional Neuroanatomy Heidelberg University  69120 Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres","family":"J\u00e4schke","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Molecular Biotechnology (IPMB) Heidelberg University  69120 Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5284-3884","authenticated-orcid":false,"given":"Murat","family":"Sunbul","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Molecular Biotechnology (IPMB) Heidelberg University  69120 Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,9]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-021-00038-x"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.6b00667"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127344"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200802376"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl103427w"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200804998"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb800025k"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt765"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1991"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2835"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.0c02620"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-01188-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.9b03736"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201806482"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1360"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2179"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-018-0105-0"},{"key":"e_1_2_8_18_1","first-page":"2027.968578","volume":"2020","author":"Wang J.","year":"2020","journal-title":"BioRxiv"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1207339"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja508478x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0249-1"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb500499x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-00794-3"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0090-6"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-022-01228-8"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39611-1"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1264"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2015.04.018"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2009.01.004"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.26369"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.11.118"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/B920865H"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b04211"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300354"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.052944.115"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b02697"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0515-7"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15362-1"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14731-0"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3528"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep34486"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-22116-0"},{"key":"e_1_2_8_43_1","author":"Askari A.","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c11969"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg595"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp250"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.52.35000"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.11.030"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22260"},{"key":"e_1_2_8_50_1","first-page":"319","volume":"449","author":"Wimmer V. C.","year":"2004","journal-title":"Pfl\u00fcgers Archiv"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-01018-x"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6NR01495J"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn4009388"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.33.000156"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2224"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq166"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1264"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.71"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-01188-6"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-021-00775-w"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001348200"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202412810","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.202412810","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202412810","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T16:54:16Z","timestamp":1788627256000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.202412810"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,9]]},"references-count":63,"journal-issue":{"issue":"51","published-print":{"date-parts":[[2024,12,16]]}},"alternative-id":["10.1002\/anie.202412810"],"URL":"https:\/\/doi.org\/10.1002\/anie.202412810","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.01.26.577321","asserted-by":"object"}]},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,9]]},"assertion":[{"value":"2024-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202412810"}}