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However, the conversion efficiency depends on the energy yield of the substrate. In this study, we developed an electro\u2010biocatalytic system by integrating electrochemical and microbial processes to upcycle CO\n                    <jats:sub>2<\/jats:sub>\n                    into a valuable product (ectoine) using renewable energy. This system initiates the electrocatalytic reduction of CO\n                    <jats:sub>2<\/jats:sub>\n                    to methane, an energy\u2010dense molecule, which then serves as an electrofuel to energize the growth of an engineered methanotrophic cell factory for ectoine biosynthesis. The scalability of this system was demonstrated using an array of ten 25\u2005cm\n                    <jats:sup>2<\/jats:sup>\n                    electrochemical cells equipped with a high\u2010performance carbon\u2010supported isolated copper catalyst. The system consistently generated methane at the cathode under a total partial current of approximately \u221237\u2005A (~175\u2005mmol\n                    <jats:sub>CH4<\/jats:sub>\n                    \u2009h\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and O\n                    <jats:sub>2<\/jats:sub>\n                    at the anode under a total partial current of approximately 62\u2005A (~583\u2005mmol\n                    <jats:sub>O2<\/jats:sub>\n                    \u2009h\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). This output met the requirements of a 3\u2010L bioreactor, even at maximum CH\n                    <jats:sub>4<\/jats:sub>\n                    and O\n                    <jats:sub>2<\/jats:sub>\n                    consumption, resulting in the high\u2010yield conversion of CO\n                    <jats:sub>2<\/jats:sub>\n                    to ectoine (1146.9\u2005mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). This work underscores the potential of electrifying the biosynthesis of valuable products from CO\n                    <jats:sub>2<\/jats:sub>\n                    , providing a sustainable avenue for biomanufacturing and energy storage.\n                  <\/jats:p>","DOI":"10.1002\/anie.202415445","type":"journal-article","created":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T01:05:35Z","timestamp":1729040735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Scalable Electro\u2010Biosynthesis of Ectoine from Greenhouse Gases"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1493-9089","authenticated-orcid":false,"given":"Shuqi","family":"Guo","sequence":"first","affiliation":[{"name":"Xi'an Key Laboratory of C1 Compound Bioconversion Technology, School of Chemical Engineering and Technology Xi'an Jiaotong University  Xi'an 710049 P. R. 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