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Here, we develop a genetically encoded live\u2010cell RNA methylation sensor that can dynamically monitor RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A level at single\u2010cell resolution. The sensor senses RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A in cells via affinity\u2010induced cytoplasmic retention using a nuclear location sequence\u2010fused m\n                    <jats:sup>6<\/jats:sup>\n                    A reader. It allows for simultaneously measure RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A dynamics and viral invasion at single\u2010cell level. Based on the single\u2010cell analytical tool, we found that SARS\u2010CoV\u20102 infection enhances host\u2010cell RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A level, and high\u2010level RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A modification in host cells, in turn, facilitates viral infection. Particularly, Omicron, a variant of SARS\u2010CoV\u20102, that features as high infection capacity, however, exhibits a reduced facilitation of m\n                    <jats:sup>6<\/jats:sup>\n                    A modification in host cells. In addition, the sensor can estimate viral inhibition via measuring cellular m\n                    <jats:sup>6<\/jats:sup>\n                    A level, that was explored for screening potential antiviral drugs. The methylation sensor can serve for elucidating the interplay between pathogens and host\u2010cell epigenetics at single\u2010cell level.\n                  <\/jats:p>","DOI":"10.1002\/anie.202418003","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T09:24:14Z","timestamp":1737624254000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dynamic, Single\u2010cell Monitoring of RNA Modifications Response to Viral Infection Using a Genetically Encoded Live\u2010cell RNA Methylation Sensor"],"prefix":"10.1002","volume":"64","author":[{"given":"Ting","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Biomass Science and Engineering Sichuan University  Chengdu 610065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Biomass Science and Engineering Sichuan University  Chengdu 610065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanwei","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Targeted Tracer Research and Development Laboratory West China Hospital Sichuan University  Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biomass Science and Engineering Sichuan University  Chengdu 610065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biomass Science and Engineering Sichuan University  Chengdu 610065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianglin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Targeted Tracer Research and Development Laboratory West China Hospital Sichuan University  Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuhan","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Biomass Science and Engineering Sichuan University  Chengdu 610065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Green Chemistry and Technology of Ministry of Education College of Chemistry Sichuan University  Chengdu 610064 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9094-124X","authenticated-orcid":false,"given":"Ruijie","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Biomass Science and Engineering Sichuan University  Chengdu 610065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,5]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.132"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1266"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.03.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.014"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12730"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.09.019"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.687"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00450-x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01243-z"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2015.11.007"},{"key":"e_1_2_8_11_1","volume":"35","author":"Li N.","year":"2021","journal-title":"Cell Rep."},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.047"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2021.100724"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5AN01797A"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-022-00765-9"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-023-00937-1"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11713-9"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01487-9"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01587-6"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-00949-w"},{"key":"e_1_2_8_21_1","author":"Huang S.","year":"2024","journal-title":"Nat. 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