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However, the biosynthetic pathway and regulatory functions of clostridial AIPs remain inadequately characterized. In this study, we employed chemical quantification, genetic investigations, and in vitro reconstitution experiments to elucidate the native\n                    <jats:italic>Ca<\/jats:italic>\n                    \u2010AIP in\n                    <jats:italic>Clostridium acetobutylicum<\/jats:italic>\n                    , a prominent industrial producer of acetone, butanol, and ethanol. Our findings identified a signal peptidase (Cac1760) and two CAAX metalloproteases (Cac0077 and Cac2478) as key players in N\u2010terminal cleavage, while AgrB was found to be essential for C\u2010terminal processing during\n                    <jats:italic>Ca<\/jats:italic>\n                    \u2010AIP biosynthesis. Notably, overexpression of\n                    <jats:italic>agrBD<\/jats:italic>\n                    led to a 4.4\u2010fold enhancement in\n                    <jats:italic>Ca<\/jats:italic>\n                    \u2010AIP formation, which corresponded with an increase in butanol production from 12.5 to 14.9\u2005g\/L, while preserving vegetative cell morphology. The direct involvement of\n                    <jats:italic>Ca<\/jats:italic>\n                    \u2010AIP in both butanol production and maintenance of cell morphology was further validated through exogenous supplementation. Collectively, these results provide novel insights into the biosynthesis of AIPs and propose a promising strategy for optimizing microbial processes in industrial applications.\n                  <\/jats:p>","DOI":"10.1002\/anie.202500904","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T12:49:36Z","timestamp":1739278176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Elucidating the Biosynthesis and Function of an Autoinducing Peptide in\n                    <i>Clostridium acetobutylicum<\/i>"],"prefix":"10.1002","volume":"64","author":[{"given":"Chao","family":"Zhu","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Bio-Intelligent Manufacturing School of Bioengineering Dalian University of Technology  Dalian 116024 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