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Another related subclass of type\u2010III copper proteins, tyrosinases, closely resemble\n                    <jats:italic>o<\/jats:italic>\n                    \u2010aminophenol oxidases structurally and enzymatically but lack their unique ability to oxidize\n                    <jats:italic>o<\/jats:italic>\n                    \u2010aminophenols into nitrosophenols. To unpuzzle the catalytic disparities of both subclasses, highly conserved amino acid residues in the vicinity of the catalytic center were identified. Notably, the Asn43 residue in the\n                    <jats:italic>o<\/jats:italic>\n                    \u2010aminophenol oxidase from\n                    <jats:italic>Streptomyces griseus<\/jats:italic>\n                    (\n                    <jats:italic>Sg<\/jats:italic>\n                    GriF) plays a pivotal role in its nitroso\u2010forming activity. Mutating the Asn43 residue in\n                    <jats:italic>Sg<\/jats:italic>\n                    GriF to isoleucine, which is present at the homologous position Ile42 in the tyrosinase from\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    sp. ZL\u201024 (\n                    <jats:italic>Sz<\/jats:italic>\n                    TYR), resulted in the loss of the nitroso\u2010forming activity in\n                    <jats:italic>Sg<\/jats:italic>\n                    GriF. Conversely, exchanging Ile42 in\n                    <jats:italic>Sz<\/jats:italic>\n                    TYR to asparagine generates nitroso\u2010forming activity in\n                    <jats:italic>Sz<\/jats:italic>\n                    TYR. The results presented herein demonstrate the feasibility of converting an\n                    <jats:italic>o<\/jats:italic>\n                    \u2010aminophenol oxidase into a tyrosinase and vice versa through a single amino acid mutation, underscoring the potential of these findings for future applications in medicinal and material sciences.\n                  <\/jats:p>","DOI":"10.1002\/anie.202501560","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T22:42:20Z","timestamp":1741732940000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of an Activity Selector for the Nitroso\u2010Forming Activity in Bacterial Type\u2010III Copper Enzymes"],"prefix":"10.1002","volume":"64","author":[{"given":"Hoa","family":"Le Xuan","sequence":"first","affiliation":[{"name":"Fakult\u00e4t f\u00fcr Chemie, Institut f\u00fcr Biophysikalische Chemie Universit\u00e4t Wien  Josef\u2010Holaubek Platz 2 Vienna 1090 Austria"},{"name":"Vienna Doctoral School in Chemistry (DoSChem) Universit\u00e4t Wien  W\u00e4hringerstra\u00dfe 42 Vienna 1090 Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Panis","sequence":"additional","affiliation":[{"name":"Fakult\u00e4t f\u00fcr Chemie, Institut f\u00fcr Biophysikalische Chemie Universit\u00e4t Wien  Josef\u2010Holaubek Platz 2 Vienna 1090 Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Rompel","sequence":"additional","affiliation":[{"name":"Fakult\u00e4t f\u00fcr Chemie, Institut f\u00fcr Biophysikalische Chemie Universit\u00e4t Wien  Josef\u2010Holaubek Platz 2 Vienna 1090 Austria"},{"name":"Vienna Doctoral School in Chemistry (DoSChem) Universit\u00e4t Wien  W\u00e4hringerstra\u00dfe 42 Vienna 1090 Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,24]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400327t"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0162-0134(95)97803-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208718109"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2006.08.006"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40828-024-00195-y"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202008859"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202000647"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202400982"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505806200"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00400a011"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.418"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.20.9721"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.52.1108"},{"key":"e_1_2_6_14_1","unstructured":"S.Omura H.Tomoda Y.Takahashi U.S. Patent USA6 512 008 2003."},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12060752"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01630a034"},{"key":"e_1_2_6_17_1","first-page":"1919","volume":"2","author":"Nishino H.","year":"1999","journal-title":"J. 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