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Here, we report a new strategy of low\u2010energy visible light\u2010induced desulfurization (LEnVLD) that enables superfast and clean protein desulfurization (half life =\u00a01.7\u00a0s) with improved reaction selectivity compared to the previous methods. The LEnVLD method can be easily carried out under very mild conditions using only a catalytic amount of fluorescent dye and household flashlight (ca. 5\u00a0W) irradiation, eliminating the need for any pyrophoric reagent, thiol additives, or excessive radical initiators, and its practicality was demonstrated by the fast and high\u2010yielding desulfurization of more than 30 peptide and protein substrates bearing a variety of sensitive functional groups (e.g., Thz, N\u2010alkylated maleimide, and thioester). Moreover, the convenience and robustness of LEnVLD enable its extension to versatile reaction scenarios (e.g., solid\u2010supported desulfurization and flow chemistry\u2010based desulfurization) that would enhance the practical capability of chemical protein synthesis.<\/jats:p>","DOI":"10.1002\/anie.202502884","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T12:18:41Z","timestamp":1744028321000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Superfast Protein Desulfurization Triggered by Low\u2010Energy Visible Light"],"prefix":"10.1002","volume":"64","author":[{"given":"Dongyang","family":"Han","sequence":"first","affiliation":[{"name":"New Cornerstone Science Laboratory Tsinghua\u2010Peking Joint Center for Life Sciences MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Center for Synthetic and Systems Biology Department of Chemistry Tsinghua 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