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However, phototoxicity often compromises imaging reliability. Here, we develop\n                    <jats:bold>Aze\u2010HMSiR<\/jats:bold>\n                    , a spontaneously blinking silicon rhodamine probe with near\u2010infrared excitation, enabling low\u2010phototoxicity, long\u2010term (50 min) SMLM imaging of lysosomal morphology and pH dynamics. This probe provides super\u2010resolution insights into lysosomal distribution, size, and lumen pH, essential for understanding their physiological and pathological roles. Using\n                    <jats:bold>Aze\u2010HMSiR<\/jats:bold>\n                    , we investigate lysosomal function under acidosis, starvation, and anticancer drug treatments. Among seven tested drugs, periplocoside significantly reduced lysosomal size, while paclitaxel increased pH and altered lysosomal distribution. These findings highlight\n                    <jats:bold>Aze\u2010HMSiR<\/jats:bold>\n                    as a powerful tool for lysosomal functional diagnostics and drug screening, offering a robust platform for studying lysosomal dynamics in response to physiological and pharmacological perturbations, particularly in cancer therapy.\n                  <\/jats:p>","DOI":"10.1002\/anie.202503177","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T03:28:41Z","timestamp":1741750121000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Whole\u2010Cell Lysosome SMLM Imaging as Indicators for Functional Diagnostics with a Low\u2010Phototoxic Spontaneously Blinking Probe"],"prefix":"10.1002","volume":"64","author":[{"given":"Qinglong","family":"Qiao","sequence":"first","affiliation":[{"name":"Dalian Institute of Chemical Physics Chinese Academy of Sciences  457 Zhongshan Road Dalian 116023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aoxuan","family":"Song","sequence":"additional","affiliation":[{"name":"Dalian Institute of Chemical Physics Chinese Academy of Sciences  457 Zhongshan Road Dalian 116023 China"},{"name":"University of Chinese Academy of Sciences  Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Fluorescence Research Group Singapore University of Technology and Design  Somapah Road Tampines 487372 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Dalian Institute of Chemical Physics Chinese Academy of Sciences  457 Zhongshan Road Dalian 116023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyan","family":"Ruan","sequence":"additional","affiliation":[{"name":"Dalian Institute of Chemical Physics Chinese Academy of Sciences  457 Zhongshan Road Dalian 116023 China"},{"name":"University of Chinese Academy of Sciences  Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhao","family":"Jia","sequence":"additional","affiliation":[{"name":"Dalian Institute of Chemical Physics Chinese Academy of Sciences  457 Zhongshan Road Dalian 116023 China"},{"name":"University of Chinese Academy of Sciences  Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaogang","family":"Liu","sequence":"additional","affiliation":[{"name":"Fluorescence Research Group Singapore University of Technology and Design  Somapah Road Tampines 487372 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaochao","family":"Xu","sequence":"additional","affiliation":[{"name":"Dalian Institute of Chemical Physics Chinese Academy of Sciences  457 Zhongshan Road Dalian 116023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"e_1_2_7_1_1","first-page":"101","volume-title":"Goodman's Medical Cell Biology","author":"Goodman S. 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