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However, their specialized metabolites, including niche and virulence factors, remained unknown. Through genome mining of environmental and clinical isolates of diverse\n                    <jats:italic>Pandoraea<\/jats:italic>\n                    species, we identified a highly conserved biosynthesis gene cluster (\n                    <jats:italic>pan<\/jats:italic>\n                    ) that codes for a nonribosomal peptide synthetase (NRPS) assembling a new siderophore. Using bioinformatics\u2010guided metabolic profiling of wild type and a targeted null mutant, we discovered the corresponding metabolites, pandorabactin A and B. Their structures and chelate (gallium) complexes were elucidated by a combination of chemical degradation, derivatization, NMR, and MS analysis. Metagenomics and bioinformatics of sputum samples of CF patients indicated that the presence of the\n                    <jats:italic>pan<\/jats:italic>\n                    gene locus correlates with the prevalence of specific bacteria in the lung microbiome. Bioassays and mass spectrometry imaging showed that pandorabactins have antibacterial activities against various lung pathogens (\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    , and\n                    <jats:italic>Stenotrophomonas<\/jats:italic>\n                    ) through depleting iron in the competitors. Taken together, these findings offer first insight into niche factors of\n                    <jats:italic>Pandoraea<\/jats:italic>\n                    and indicate that pandorabactins shape the diseased lung microbiota through the competition for iron.\n                  <\/jats:p>","DOI":"10.1002\/anie.202505714","type":"journal-article","created":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T14:37:48Z","timestamp":1743691068000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Antibacterial Siderophores of\n                    <i>Pandoraea<\/i>\n                    Pathogens and Their Impact on the Diseased Lung Microbiota"],"prefix":"10.1002","volume":"64","author":[{"given":"Elena","family":"Herzog","sequence":"first","affiliation":[{"name":"Department of Biomolecular Chemistry Leibniz Institute for Natural Product Research and Infection Biology (HKI)  Beutenbergstra\u00dfe 11a 07745 Jena 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