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To address these challenges, we generated a library of structurally diverse sialyl glycosides containing 4\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010derivatized sialic acids by a highly efficient one\u2010pot two\u2010enzyme chemoenzymatic sialylation strategy. Sialosides containing 4\u2010azido\u2010substituted sialic acid were selectively cleaved by sialidases from influenza viruses, whereas sialosides containing 4\u2010acetamido\u2010modified sialic acid were resistant to sialidase cleavage. Interestingly, sialosides containing 4\u2010amino\u2010 or 4\u2010guanidino\u2010substituted sialic acid were effective inhibitors moderately or highly resistant to cleavage by influenza sialidases (also called neuraminidases). The sialosides containing the 4\u2010guanidino\u2010substituted sialic acid represent a new class of sialidase substrate analog\u2010based inhibitors. We took advantage of this unique property to create a ligand\u2010based approach for efficiently isolating influenza virions from egg allantoic fluid with high purity. Together, these compounds are versatile probes and ligands for developing new approaches to detect, profile, isolate, and characterize influenza viruses via neuraminidases on their surface.\n                  <\/jats:p>","DOI":"10.1002\/anie.202505903","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T22:32:06Z","timestamp":1744324326000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Catch, Cut, or Block? Versatile 4\u2010\n                    <i>N<\/i>\n                    \u2010Derivatized Sialyl Glycosides for Influenza Virus Neuraminidase Detection and Purification"],"prefix":"10.1002","volume":"64","author":[{"given":"Yue","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Chemistry University of California  One Shields Avenue Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of California  One Shields Avenue Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand Kumar","family":"Agrahari","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of California  One Shields Avenue Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Gao","sequence":"additional","affiliation":[{"name":"Division of Viral Products Center for Biologics Evaluation and Research Food and Drug Administration  Silver Spring MD 20993 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeog","family":"Kang","sequence":"additional","affiliation":[{"name":"Division of Viral Products Center for Biologics Evaluation and Research Food and Drug Administration  Silver Spring MD 20993 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Daniels","sequence":"additional","affiliation":[{"name":"Division of Viral Products Center for Biologics Evaluation and Research Food and Drug Administration  Silver Spring MD 20993 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of California  One Shields Avenue Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,21]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1177\/00333549101250S305"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-017-0918-y"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13337-022-00800-z"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.adj2379"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2018.10.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.00175-24","volume":"15","author":"Li Y.","year":"2024","journal-title":"mBio"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a038778"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607614103"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy103"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/irv.13276"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02332-17"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv195"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz639"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis935"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00417-16"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2016.10.001"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay0678"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12706"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10143"},{"key":"e_1_2_7_20_1","first-page":"487","volume":"37","author":"Peteranderl C.","year":"2016","journal-title":"Crit. 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