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However, their selective synthesis remains a considerable challenge in catalysis. Regioselective hydration of non\u2010activated C\u2500C double bonds is typically hindered by poor selectivity and low atom efficiency in conventional methods. In this study, we harness the underexplored potential of the acyclic carotenoid 1,2\u2010hydratase from\n                    <jats:italic>Rubrivivax gelatinosus<\/jats:italic>\n                    IL144, employing it as a whole\u2010cell biocatalyst for cofactor\u2010independent terminal hydration of a diverse range of terpenes. This enzyme demonstrates exceptional activity across more than 20 C\n                    <jats:sub>12<\/jats:sub>\n                    \u2500C\n                    <jats:sub>20<\/jats:sub>\n                    terpenes and shows notable tolerance to various functional groups, establishing it as a valuable tool for sustainable organic synthesis. We emphasize the critical influence of expression system choice in maximizing enzymatic performance, enabling high\u2010yield transformations on the gram scale. Through a combination of homology modeling, consensus analysis, and targeted mutagenesis, essential residues involved in catalytic activity were identified. Notably, enhanced catalytic efficiency was only achievable through the epistatic effect of three specific mutations. These findings highlight the biocatalytic potential of acyclic carotenoid hydratase, offering a green and efficient route to the production of valuable tertiary alcohols.\n                  <\/jats:p>","DOI":"10.1002\/anie.202505942","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T13:58:59Z","timestamp":1751983139000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Regioselective Hydration of Terpenes with Cofactor\u2010Independent Carotenoid 1,2\u2010Hydratase"],"prefix":"10.1002","volume":"64","author":[{"given":"Philip","family":"Horz","sequence":"first","affiliation":[{"name":"Department of Technical Biochemistry Institute of Biochemistry and Technical Biochemistry Department of Technical Biochemistry University of Stuttgart  Allmandring 31 70569 Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"H\u00e4rterich","sequence":"additional","affiliation":[{"name":"Department of Technical Biochemistry Institute of Biochemistry and Technical Biochemistry Department of Technical Biochemistry University of Stuttgart  Allmandring 31 70569 Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Technical Biochemistry Institute of Biochemistry and Technical Biochemistry Department of Technical Biochemistry University of Stuttgart  Allmandring 31 70569 Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas D.","family":"Travnicek","sequence":"additional","affiliation":[{"name":"Department of Technical Biochemistry Institute of Biochemistry and Technical Biochemistry Department of Technical Biochemistry University of Stuttgart  Allmandring 31 70569 Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina M.","family":"Nestl","sequence":"additional","affiliation":[{"name":"Innophore GmbH  Am Eisernen Tor 3 8010 Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Kahler","sequence":"additional","affiliation":[{"name":"Innophore GmbH  Am Eisernen Tor 3 8010 Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6259-3348","authenticated-orcid":false,"given":"Bernhard","family":"Hauer","sequence":"additional","affiliation":[{"name":"Department of Technical Biochemistry Institute of Biochemistry and Technical Biochemistry Department of Technical Biochemistry University of Stuttgart  Allmandring 31 70569 Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202005719"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ie50338a020"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01141a046"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01480a038"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00748a043"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2009.08.128"},{"key":"e_1_2_5_7_1","unstructured":"J. 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