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Transforming CO\/CO\n                    <jats:sub>2<\/jats:sub>\n                    , a prevalent anthropogenic air pollutant, into valuable carbon chemicals either directly or through intermediate steps via biocatalytic methods offers a promising pathway to achieve net\u2010zero emissions across industries and the environment. However, completely eliminating oxygen from industrial biotransformations, especially under ambient conditions, is exceedingly onerous. Here, we engineered variants of the CODH2 from\n                    <jats:italic>Carboxydothermus hydrogenoformans<\/jats:italic>\n                    (\n                    <jats:italic>Ch<\/jats:italic>\n                    CODH2) with dual blocking at both the O\n                    <jats:sub>2<\/jats:sub>\n                    entrance and near the active site, effectively sealing the tunnel against atmospheric O\n                    <jats:sub>2<\/jats:sub>\n                    levels (20%). The O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010tunnel engineered A559W\/V610H variant demonstrated a marked improvement in air stability, with a half\u2010life of 24.6\u00a0h compared to the wild type's 2.4\u00a0h. Crystallographic snapshots of this air\u2010viable variant after 24\u00a0h of exposure revealed the robust integrity of the fortified FeS and NiFeS clusters. Additionally, electro\u2010enzymatic reactions corroborated its CO\/CO\n                    <jats:sub>2<\/jats:sub>\n                    conversion capability even in ubiquitous air. These findings, which address the O\n                    <jats:sub>2<\/jats:sub>\n                    sensitivity of anaerobic enzymes caused by O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced metal cluster collapse, enhance their potential for biological CO\/CO\n                    <jats:sub>2<\/jats:sub>\n                    transformations in O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010rich environments, thereby broadening their industrial viability and applicability.\n                  <\/jats:p>","DOI":"10.1002\/anie.202508565","type":"journal-article","created":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T11:53:55Z","timestamp":1749038035000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Fortification of FeS Clusters Reshapes Anaerobic CO Dehydrogenase into an Air\u2010Viable Enzyme Through Multilayered Sealing of O\n                    <sub>2<\/sub>\n                    Tunnels"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1264-7250","authenticated-orcid":false,"given":"Suk Min","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biotechnology The Catholic University of Korea  Bucheon 14662 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So Yeon","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Natural Sciences Seoul National University  Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingu","family":"Kang","sequence":"additional","affiliation":[{"name":"School of Energy and Chemical Engineering Ulsan National Institute of Science and Technology (UNIST)  Ulsan 44919 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Seok","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Natural Sciences Seoul National University  Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Heuck","family":"Kang","sequence":"additional","affiliation":[{"name":"School of Energy and Chemical Engineering Ulsan National Institute of Science and Technology (UNIST)  Ulsan 44919 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye\u2010Jin","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Natural Sciences Seoul National University  Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunwoo","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Energy and Chemical Engineering Ulsan National Institute of Science and Technology (UNIST)  Ulsan 44919 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jungki","family":"Ryu","sequence":"additional","affiliation":[{"name":"School of Energy and Chemical Engineering Ulsan National Institute of Science and Technology (UNIST)  Ulsan 44919 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Natural Sciences Seoul National University  Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4729-693X","authenticated-orcid":false,"given":"Yong Hwan","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Energy and Chemical Engineering Ulsan National Institute of Science and Technology (UNIST)  Ulsan 44919 Republic of Korea"},{"name":"Graduate School of Carbon Neutrality Ulsan National Institute of Science and Technology (UNIST)  Ulsan 44919 Republic 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