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Inspired by natural chloroplasts and mitochondria, we build an innovative polydopamine\u2010armored multiple enzyme microreactor for co\u2010immobilizing glyceraldehyde\u20103\u2010phosphate dehydrogenase (GAPDH), 3\u2010phosphoglyceric phosphokinase (PGK), formate dehydrogenase (FDH), and ATPase\u2010incorporating proteoliposome, providing spatially confined microenvironments akin to natural systems. Within this microreactor, GAPDH and PGK catalyze the conversion of glyceraldehyde 3\u2010phosphate to 3\u2010phosphoglyceric acid, reducing \u03b2\u2010nicotinamide adenine dinucleotide (NAD\n                    <jats:sup>+<\/jats:sup>\n                    ) to NADH and generating a proton influx that drives ATP synthesis. The microreactor possesses strong affinity for CO\n                    <jats:sub>2<\/jats:sub>\n                    , combined with FDH, facilitates the reduction of CO\n                    <jats:sub>2<\/jats:sub>\n                    to formic acid, oxidizing NADH back to NAD\n                    <jats:sup>+<\/jats:sup>\n                    and enabling the recycling of the NAD\n                    <jats:sup>+<\/jats:sup>\n                    \/NADH redox couple. This process further boosts ATP production by contributing additional protons. Such microreactor adeptly orchestrates the chloroplast's enzymes to fix CO\n                    <jats:sub>2<\/jats:sub>\n                    and the mitochondrion's enzymes to synthesize ATP into a unified artificial biomimetic system, effectively replicating the glycolysis process to simultaneously achieve CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation, NADH regeneration, and ATP synthesis. This strategy not only holds great potential to inspire significant design innovations for more efficient ATP synthesis from low\u2010value substances but also greatly expands the application scenarios for biomolecular motors.\n                  <\/jats:p>","DOI":"10.1002\/anie.202509835","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T10:49:27Z","timestamp":1750848567000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Reduction of CO\n                    <sub>2<\/sub>\n                    Accompanying ATP Synthesis in Polydopamine Microreactors Covered by Lipid Bilayers with ATPase"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6952-5206","authenticated-orcid":false,"given":"Yang","family":"Xu","sequence":"first","affiliation":[{"name":"Beijing National Laboratory for Molecular Sciences (BNLMS) CAS Key Lab of Colloid Interface and 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanze","family":"Meng","sequence":"additional","affiliation":[{"name":"Beijing National Laboratory for Molecular Sciences (BNLMS) CAS Key Lab of Colloid Interface and Chemical Thermodynamics Institute of Chemistry Chinese Academy of Sciences  Beijing 100190 China"},{"name":"University of Chinese Academy of Sciences  Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Shu","sequence":"additional","affiliation":[{"name":"Beijing National Laboratory for Molecular Sciences (BNLMS) CAS Key Lab of Colloid Interface and Chemical Thermodynamics Institute of Chemistry Chinese Academy of Sciences  Beijing 100190 China"},{"name":"University of Chinese Academy of Sciences  Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Xi'an Shiyou University  Xi'an 710065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zibo","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences Jilin University  Changchun 130012 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9575-3125","authenticated-orcid":false,"given":"Junbai","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing National Laboratory for Molecular Sciences (BNLMS) CAS Key Lab of Colloid Interface and Chemical Thermodynamics Institute of Chemistry Chinese Academy of Sciences  Beijing 100190 China"},{"name":"University of Chinese Academy of Sciences  Beijing 100049 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