{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T02:34:20Z","timestamp":1783132460776,"version":"3.54.6"},"reference-count":68,"publisher":"Wiley","issue":"34","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000736","name":"University of East Anglia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000736","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2025,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Iron is in an essential micronutrient in living systems. However, it is also potentially toxic and so the concentration of chelatable iron within cells is tightly regulated to prevent catalytic formation of harmful reactive oxygen species (ROS). Ferritins play a key role in iron homeostasis by storing excess iron as an insoluble ferric mineral within the protein. When bacterial cells become iron deficient, this store may be accessed by reduction\/solubilisation of the iron. Bacterioferritins utilise heme, bound at an inter\u2010subunit site, to support electron transfer to the stored mineral. Electrons for heme reduction are shuttled from NADPH via Bfd, a [2Fe\u20132S] cluster\u2010containing ferredoxin. This raises the paradox that the synthesis of an iron\u2010dependent protein co\u2010factor is required under conditions of iron\u2010deficiency so that stored iron can be utilised. Here, we show that exposure of Bfd to ROS suppresses the capacity of the protein to stimulate iron release from bacterioferritin. We propose that reliance of iron release on Bfd evolved to ensure that chelatable iron levels do not increase under oxidative stress conditions. Thus, the Bfd iron\u2013sulfur cluster functions as a \u201cbiological fuse\u201d in providing a fail\u2010safe that immediately halts iron release once ROS accumulate to damaging concentrations.<\/jats:p>","DOI":"10.1002\/anie.202511340","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T12:33:54Z","timestamp":1750250034000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Iron\u2013Sulfur Cluster of Bacterioferritin\u2010Associated Ferredoxin (Bfd): a \u201cBiological Fuse\u201d that Prevents Oxidative Damage to Cells?"],"prefix":"10.1002","volume":"64","author":[{"given":"Justin M.","family":"Bradley","sequence":"first","affiliation":[{"name":"Centre for Molecular and Structural Biochemistry School of Chemistry University of East Anglia  Norwich Research Park Norwich NR4 7TJ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiden M.","family":"Carter","sequence":"additional","affiliation":[{"name":"Centre for Molecular and Structural Biochemistry School of Chemistry University of East Anglia  Norwich Research Park Norwich NR4 7TJ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zinnia","family":"Bugg","sequence":"additional","affiliation":[{"name":"Centre for Molecular and Structural Biochemistry School of Chemistry University of East Anglia  Norwich Research Park Norwich NR4 7TJ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon C.","family":"Andrews","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of Reading, Whiteknights  Reading RG6 6EX UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9780-4061","authenticated-orcid":false,"given":"Nick E.","family":"Le Brun","sequence":"additional","affiliation":[{"name":"Centre for Molecular and Structural Biochemistry School of Chemistry University of East Anglia  Norwich Research Park Norwich NR4 7TJ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,25]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2016.11.007"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr040653o"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400415k"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007750050002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5326.653"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01597a021"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1518"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3032"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00128-18"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV120.007746"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-006-0125-6"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2010.03.019"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9DT03570B"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.669713"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.623082"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ic3010135"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7MT00008A"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.08.017"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1318417111"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2017.02.027"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202000064"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi401517f"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c01337"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-024-02058-w"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2018.02.011"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-021-01850-2"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00612a021"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1430311"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00430a033"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi002509c"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00648-1"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja305180n"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202401379"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444997006811"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.175034"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.0c00669"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7MT00042A"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b00937"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0357053"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-011-9476-8"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7057"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.13635"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05439.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1707189114"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202015964"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-794-5_4"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi900561a"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2024.119772"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2010.04.002"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1306.001"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12410"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.11.5710"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2009.11.019"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.15.4639-4643.1999"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.15.4571-4579.2001"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1mt00022e"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05701.x"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00092-09"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00083.x"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6087"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4SC04618H"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7SC02801F"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.8b00823"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2021.111407"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-021-01854-y"},{"key":"e_1_2_9_66_1","first-page":"641","volume":"21","author":"Schwarz K. B.","year":"1996","journal-title":"Biol. Med."},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2802"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-021-00882-0"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202511340","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.202511340","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202511340","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T18:15:10Z","timestamp":1775844910000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.202511340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,25]]},"references-count":68,"journal-issue":{"issue":"34","published-print":{"date-parts":[[2025,8,18]]}},"alternative-id":["10.1002\/anie.202511340"],"URL":"https:\/\/doi.org\/10.1002\/anie.202511340","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,25]]},"assertion":[{"value":"2025-05-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202511340"}}