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However, monitoring nucleolar viscosity in living cells remains a great challenge, which also leads to the lack of a clear report on the relationship between nucleolar viscosity and nucleolar stress. Herein, a nucleolus\u2010targeted platinum(II) complex with monodentate ligands (\n                    <jats:bold>Pt2<\/jats:bold>\n                    ) has been developed, which can rapidly accumulate in the nucleolus and inhibit ribosome biogenesis through energy\u2010dependent signaling pathways, eventually inducing nucleolar stress and simultaneously monitoring nucleolar viscosity via phosphorescence lifetime imaging (PLIM). The phosphorescence response of\n                    <jats:bold>Pt2<\/jats:bold>\n                    to viscosity is achieved by suppressing the structural distortion to the non\u2010emissive d\u2013d excited state and restricting the rotation of Pt\u2500N coordination bonds, which is different from other commonly reported viscosity probes in the literature. After exposure to\n                    <jats:bold>Pt2<\/jats:bold>\n                    or other antineoplastic agents, which can induce nucleolar stress, the gradual decrease of nucleolar viscosity is observed in living cells in a quantitative and real\u2010time manner, indicating that nucleolar viscosity can be used as a real\u2010time monitor of the extent of nucleolar stress. This work reports the first example of a metal complex that can achieve simultaneous induction and self\u2010reporting of nucleolar stress via PLIM of nucleolar viscosity.\n                  <\/jats:p>","DOI":"10.1002\/anie.202512465","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:53:12Z","timestamp":1761663192000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Simultaneous Induction and Self\u2010Reporting of Nucleolar Stress by a Nucleolus\u2010Targeted Platinum(II) Complex via Lifetime Imaging"],"prefix":"10.1002","volume":"64","author":[{"given":"Bin","family":"Liu","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Bioinorganic and Synthetic Chemistry State Key Laboratory of Anti\u2010Infective Drug Discovery and Development Institute of Green Chemistry and Molecular Engineering Guangdong Basic Research Center of Excellence for Functional Molecular 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Republic of China"},{"name":"State Key Laboratory of Coordination Chemistry Nanjing University  Nanjing 210023 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8349-4429","authenticated-orcid":false,"given":"Vivian Wing\u2010Wah","family":"Yam","sequence":"additional","affiliation":[{"name":"Institute of Molecular Functional Materials State Key Laboratory of Synthetic Chemistry and Department of Chemistry The University of Hong Kong  Pokfulam Road Hong Kong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4291"},{"key":"e_1_2_9_2_1","first-page":"313","volume":"23","author":"Busch H.","year":"1963","journal-title":"Cancer 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