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However, the efficient coupling of photocatalysts with multiple enzymes to enable photo\u2010enzymatic cascade catalysis remains a significant challenge. Herein, we report the construction of hydrogen\u2010bonded organic frameworks (HOFs) that integrate Ru\u2010based photocatalysts with three\u2010enzyme cascades of formate dehydrogenase (FDH), formaldehyde dehydrogenase (FaldDH), and alcohol dehydrogenase (ADH) via in situ co\u2010assembly in water. The\n                    <jats:bold>RuHOF<\/jats:bold>\n                    exhibits exceptional nicotinamide adenine dinucleotide (NADH) photo\u2010regeneration activity (4.5\u00a0mM\u00a0h\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), while the\n                    <jats:bold>FDH@RuHOF<\/jats:bold>\n                    hybrid converts CO\n                    <jats:sub>2<\/jats:sub>\n                    to formic acid with a turnover frequency (TOF) of 681 h\n                    <jats:sup>\u22121<\/jats:sup>\n                    (238 \u00b5M\u00a0h\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) over 24 h. By engineering\n                    <jats:bold>FDH\/FaldDH\/ADH@RuHOF<\/jats:bold>\n                    ternary systems, we achieve sustained CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010to\u2010methanol conversion through photo\u2010enzymatic cascade catalysis, delivering 2.2\u00a0mM methanol production with an apparent quantum efficiency (AQY) of 5.5% (92 \u00b5M\u00a0h\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) over 24\u00a0h with 85% activity retention after five catalytic cycles. This work opens a promising avenue for the development of efficient multi\u2010enzyme cascade artificial photosynthetic systems toward steady and recyclable CO\n                    <jats:sub>2<\/jats:sub>\n                    valorization.\n                  <\/jats:p>","DOI":"10.1002\/anie.202516599","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T04:01:13Z","timestamp":1757995273000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Integration of Multiple Enzymes Within Hydrogen\u2010Bonded Organic Frameworks for Efficient Cascade Photocatalytic CO\n                    <sub>2<\/sub>\n                    \u2010to\u2010Methanol Conversion in Water"],"prefix":"10.1002","volume":"64","author":[{"given":"Jiakang","family":"Tang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis Shanghai Institute of Organic Chemistry University of Chinese Academy of Sciences Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Chemistry Fudan University  Shanghai 200438 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxuan","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis Shanghai Institute of Organic Chemistry University of Chinese Academy of Sciences Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixing","family":"Lei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis Shanghai Institute of Organic Chemistry University of Chinese Academy of Sciences Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao\u2010Yan","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis Shanghai Institute of Organic Chemistry University of Chinese Academy of Sciences Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhan\u2010Ting","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis Shanghai Institute of Organic Chemistry University of Chinese Academy of Sciences Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8339-0563","authenticated-orcid":false,"given":"Jia","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis Shanghai Institute of Organic Chemistry University of Chinese Academy of Sciences Chinese Academy of Sciences  Shanghai 200032 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