{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T21:22:34Z","timestamp":1786310554688,"version":"build-2736575974"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,1,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Current methods for sequence\u2010selective biomolecule isolation suffer from high cost, off\u2010target effects, and limited flexibility. Here, we introduce\n                    <jats:italic>LASSO<\/jats:italic>\n                    (cross\n                    <jats:bold>L<\/jats:bold>\n                    ink\u2010\n                    <jats:bold>A<\/jats:bold>\n                    ssisted\n                    <jats:bold>S<\/jats:bold>\n                    equence\u2010\n                    <jats:bold>S<\/jats:bold>\n                    elective is\n                    <jats:bold>O<\/jats:bold>\n                    lation), a versatile platform using programmable polymer phase separation to capture biomolecules under native conditions. LASSO relies on\n                    <jats:italic>combinatorial crosslinker libraries<\/jats:italic>\n                    \u2014diverse mixtures of DNA strands that collectively trigger the formation of highly swollen polymer agglomerates with near\u2010zero background binding. We demonstrate\u00a0&gt;80% pulldown efficiency for diverse targets, including DNA, SARS\u2010CoV\u20102 RNA, and human thrombin. LASSO provides 8\u201320x higher binding capacity (4\u00a0nmol mg\n                    <jats:sup>\u22121<\/jats:sup>\n                    polymer) than commercial microbeads. In RNA\u2010seq workflows, LASSO depleted ribosomal RNA with 86% efficiency, while yielding up to 7x fewer off\u2010target outliers versus state\u2010of\u2010the\u2010art magnetic beads and enzyme\u2010based methods. Thrombin was captured via switchable aptamers with 90% efficiency, and a gentle release mechanism allowed the subsequent isolation of 98% enzymatically active proteins from the polymer. LASSO's cost\u2010effectiveness ($0.96\/sample versus $46\u2013$51 for commercial kits), long\u2010term stability (7\u00a0+\u00a0years), simple usage, and modularity position it to advance diagnostics, transcriptomics, and bionanotechnology workflows.\n                  <\/jats:p>","DOI":"10.1002\/anie.202517600","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T05:25:42Z","timestamp":1766121942000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Versatile and Selective Biomolecule Pulldown with Combinatorial DNA\u2010Crosslinked Polymers"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8565-2379","authenticated-orcid":false,"given":"Sarah K.","family":"Speed","sequence":"first","affiliation":[{"name":"Division of Polymer Biomaterials Science Leibniz Institute of Polymer Research Dresden Dresden 01069 Germany"},{"name":"Faculty of Chemistry and Food Chemistry Technische Universit\u00e4t Dresden Dresden 01062 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3898-6333","authenticated-orcid":false,"given":"Krishna","family":"Gupta","sequence":"additional","affiliation":[{"name":"Division of Polymer Biomaterials Science Leibniz Institute of Polymer Research Dresden Dresden 01069 Germany"},{"name":"Faculty of Chemistry and Food Chemistry Technische Universit\u00e4t Dresden Dresden 01062 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5472-3333","authenticated-orcid":false,"given":"Yu\u2010Hsuan","family":"Peng","sequence":"additional","affiliation":[{"name":"Division of Polymer Biomaterials Science Leibniz Institute of Polymer Research Dresden Dresden 01069 Germany"},{"name":"Faculty of Chemistry and Food Chemistry Technische Universit\u00e4t Dresden Dresden 01062 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