{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T13:36:36Z","timestamp":1793194596266,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"48","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2025,11,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Designing single\u2010atom nanozymes (SAzymes) with superparamagnetism and peroxidase (POD)\u2010specific activity was still a challenge. In this research, we reported a novel magnetic SAzyme through Cu\u2010doping engineering in a mild one\u2010pot synthesis. Theoretical calculations revealed that Cu\u2010doping can decrease the\n                    <jats:italic>d<\/jats:italic>\n                    \u2010band center of NiCu substrate so that Os atoms adsorption and agglomeration can be suppressed, single\u2010atom Os can absorb on the substrate, moreover, Cu\u2010doping can bidirectionally modulate the energy barriers in the POD (decreasing) and oxidase (OXD) (increasing) catalytic process, which endows NiCu\u2500Os with superior POD\u2010activity but negligible OXD\u2010activity. Attributing to the superparamagnetism and superior POD\u2010activity, NiCu\u2500Os SAzyme can separate targets in biological matrix and catalytic colorimetric detection without pretreatment. For practical application, NiCu\u2500Os coupled strips were developed for point\u2010of\u2010care (POC) diagnosis of colorectal cancer (CRC), three biomarkers (CA19\u20109, CEA, CST\u20104) can be separated from serum and visually detected on a single strip within 20\u00a0min, achieving perfect accuracy (AUC 100%) for CRC patients\u2019 recognition. Furthermore, attributing to the POD\u2010specificity, NiCu\u2500Os enables rapid (13\u00a0min) and visual distinction of CRC tumor tissues from non\u2010tumor tissues via endogenous hydrogen peroxide levels. This work presents a novel paradigm for magnetic and POD\u2010specific SAzymes, paving the way for integrated enrichment\u2013amplification\u2013detection platforms in POC diagnostics.\n                  <\/jats:p>","DOI":"10.1002\/anie.202517723","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:34:17Z","timestamp":1760002457000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Magnetic and Peroxidase\u2010Specific Single\u2010Atom Nanozyme Through Cu\u2010Doping Engineering for Ultrasensitive and Visual Tumor Diagnosis"],"prefix":"10.1002","volume":"64","author":[{"given":"Pengyou","family":"Zhou","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of environment and aging Capital Medical University  No. 10 Xitoutiao, You An Men Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of environment and aging Capital Medical University  No. 10 Xitoutiao, You An Men Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingtao","family":"Meng","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of environment and aging Capital Medical University  No. 10 Xitoutiao, You An Men Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorui","family":"Lin","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of environment and aging Capital Medical University  No. 10 Xitoutiao, You An Men Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5269-1619","authenticated-orcid":false,"given":"Yuanfeng","family":"Pang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of environment and aging Capital Medical University  No. 10 Xitoutiao, You An Men Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Chen","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of environment and aging Capital Medical University  No. 10 Xitoutiao, You An Men Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,9]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)00360-X"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra2200869"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2024.07.005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.22231"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2304714"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c16698"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30391-8"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42889-w"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c02343"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202422588"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202300119"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.156835"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c07680"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c04378"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202400875"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-62063-8"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c09238"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202420200"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c14046"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c01618"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114446"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202212317"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2024.116044"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c05267"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202502574"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c02156"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c00008"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-57190-1"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c05503"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-021-00609-x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202402674"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/aenm.202403464"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5c00544"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.2c04235"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/aenm.202403624"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-021-00983-4"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202111999"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-018-0108-0"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202107054"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202418731"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44160-024-00685-4"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c12734"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34423-1"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/adom.202401318"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202517723","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.202517723","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202517723","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:06:43Z","timestamp":1790698003000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.202517723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,9]]},"references-count":44,"journal-issue":{"issue":"48","published-print":{"date-parts":[[2025,11,24]]}},"alternative-id":["10.1002\/anie.202517723"],"URL":"https:\/\/doi.org\/10.1002\/anie.202517723","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,9]]},"assertion":[{"value":"2025-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202517723"}}