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Mitotic kinases that regulate the proliferation of the parasites by controlling nuclear division, segregation, and cytokinesis. We evaluated the potential of human Aurora kinase (Aur) inhibitors to prevent\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    development by targeting members of the Aurora\u2010related kinase (Ark) family in this parasite. Several human AurB inhibitors exhibited multistage potency (&lt; 250\u00a0nM) against all proliferative stages of parasite development, including asexual blood stages, liver schizonts, and male gametes. The most potent compounds, hesperadin, TAE684, and AT83, exhibited\u00a0&gt;\u00a01000x selectivity towards the parasite. Importantly, we identified\n                    <jats:italic>Pf<\/jats:italic>\n                    Ark1 as the principal vulnerable Ark family member, with specific inhibition of\n                    <jats:italic>Pf<\/jats:italic>\n                    Ark1 as the primary target for hesperadin. Hesperadin's whole\u2010cell and protein activity validates it as a unique\n                    <jats:italic>Pf<\/jats:italic>\n                    Ark1 tool compound. Inhibition of\n                    <jats:italic>Pf<\/jats:italic>\n                    Ark1 results in the parasite's inability to complete mitotic processes, presenting with unsegregated, multi\u2010lobed nuclei caused by aberrant microtubule organization. This suggests\n                    <jats:italic>Pf<\/jats:italic>\n                    Ark1 is the main Aur mitotic kinase in proliferative stages of\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    , characterized by bifunctional AurA and B activity. This paves the way for drug\u2010discovery campaigns based on hesperadin targeting\n                    <jats:italic>Pf<\/jats:italic>\n                    Ark1.\n                  <\/jats:p>","DOI":"10.1002\/anie.202518493","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T05:55:26Z","timestamp":1761285326000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Targeting Aurora Kinases as Essential Cell\u2010Cycle Regulators to Deliver Multi\u2010Stage Antimalarials Against\n                    <i>Plasmodium Falciparum<\/i>"],"prefix":"10.1002","volume":"64","author":[{"given":"Henrico","family":"Langeveld","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Genetics and Microbiology  Hatfield Pretoria 0028 South Africa"},{"name":"Institute for Sustainable Malaria Control University of Pretoria  Hatfield Pretoria 0028 South 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