{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:05:35Z","timestamp":1791223535364,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Tuberculosis (TB), caused by infection with the bacterium\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    (\n                    <jats:italic>Mtb<\/jats:italic>\n                    ), remains one of the most prevalent infectious diseases worldwide. Despite decades of dedicated efforts to develop effective prevention strategies, including vaccines, TB continues to cause significant morbidity and mortality globally. Beyond the Bacille Calmette\u2013Gu\u00e9rin (BCG) vaccine, limited progress has been made to develop more effective TB vaccines. A better understanding of the immunomodulatory roles of key\n                    <jats:italic>Mtb<\/jats:italic>\n                    protein virulence factors is therefore needed for the development of more efficacious protein\u2010based vaccine candidates. Herein, we report a highly efficient method for the semi\u2010synthesis of two native mycobacterial lipoproteins and glycolipoproteins, LprA and Mpt83, respectively. Capitalising on the enhanced reactivity of peptide selenoesters as acyl donors, the 21\u201323\u00a0kDa homogeneously lipidated proteins could be generated in a single ligation step. The homogeneous mycobacterial lipoprotein and glycolipoprotein molecules were shown to be agonists of Toll\u2010like receptor 2 (TLR2) and led to the potent induction of pro\u2010inflammatory cytokines and chemokines in cells. Taken together, this work presents a robust semi\u2010synthetic platform for accessing lipoproteins involved in host\u2013pathogen interactions that can help guide future TB vaccine design.\n                  <\/jats:p>","DOI":"10.1002\/anie.202519647","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T10:43:36Z","timestamp":1762771416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Semi\u2010Synthesis of Immunogenic Mycobacterial Lipoproteins via Aryl Selenoester\u2010Mediated Expressed Protein Ligation"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8278-5363","authenticated-orcid":false,"given":"Lucas","family":"Kambanis","sequence":"first","affiliation":[{"name":"School of Chemistry The University of Sydney  Sydney New South Wales 2006 Australia"},{"name":"Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science The University of Sydney  Sydney New South Wales 2006 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua J.","family":"Dilly","sequence":"additional","affiliation":[{"name":"School of Chemistry The University of Sydney  Sydney New South Wales 2006 Australia"},{"name":"Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science The University of Sydney  Sydney New South Wales 2006 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1481-370X","authenticated-orcid":false,"given":"Joshua W. 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