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However, current surface technologies designed to achieve such dual biological functions are often limited by complex procedures and insufficient duration of efficacy. Herein, mimicking the innate immune mechanism of macrophages, wherein they eliminate pathogens through the synergistic action of nitric oxide (NO) and lysozyme secreted by them, we report a bioinspired glutathione peroxidase mimic (i.e., 1,4,7,10\u2010tetraazacyclododecane\u20101,4,7,10\u2010tetraacetic acid\u2010Cu (II)(DOTA\u2010Cu) coordination complex)\u2010engineered lysozyme (DOTA\u2010Cu\/Lyz) self\u2010assembling coating for tailoring anti\u2010thrombotic and anti\u2010infective functions. This self\u2010assembled DOTA\u2010Cu\/Lyz coating exhibits potent GPx\u2010mimicking activity, catalyzing the decomposition of endogenous S\u2010nitrosothiol into NO under blood conditions. This process synergizes with lysozyme to effectively kill bacteria, thereby mimicking the pathogen\u2010killing mechanism of macrophages. Meanwhile, the sustained release of NO inhibits platelet activation and adhesion via NO\u2010cyclic guanosine monophosphate (cGMP) signaling pathway, providing long\u2010lasting antithrombotic effects. Overall, such self\u2010assembled DOTA\u2010Cu\/Lyz coating represents a promising strategy to address the issue\u00a0of thrombosis and infection associated with blood\u2010contacting devices.<\/jats:p>","DOI":"10.1002\/anie.202519702","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T05:48:46Z","timestamp":1764395326000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mimicking Macrophage Immune Mechanism for Infection and Thrombosis Prevention on a Dual\u2010Activity Hybrid Enzyme\u2010Assembled Surface"],"prefix":"10.1002","volume":"65","author":[{"given":"Xiaohui","family":"Mou","sequence":"first","affiliation":[{"name":"The Tenth Affiliated Hospital Southern Medical University (Dongguan People's Hospital)  Dongguan Guangdong 523059 P.R. 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