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Here, we present\n                    <jats:styled-content>s<\/jats:styled-content>\n                    ingle\u2010injection\n                    <jats:styled-content>m<\/jats:styled-content>\n                    ulti\u2010omics\n                    <jats:styled-content>a<\/jats:styled-content>\n                    nalysis by\n                    <jats:styled-content>d<\/jats:styled-content>\n                    irect infusion (SMAD), an integrated platform leveraging ion mobility mass spectrometry and self\u2010developed software tools to enable single injection multi\u2010omics analysis without liquid chromatography. SMAD allows quantification of over 9000 metabolite\n                    <jats:italic>m\/z<\/jats:italic>\n                    features and over 1300 proteins from the same sample in less than 5 min. We validated the efficiency and reliability of SMAD with three case studies: (1) mouse macrophages after M1\/M2 polarization and senescence, (2) a pilot drug screen in human cells, and (3) large\u2010scale high\u2010throughput drug screening of mammalian cells in 96\u2010well plates. Finally, relationships between proteomic and metabolomic data are discovered by machine learning and validated.\n                  <\/jats:p>","DOI":"10.1002\/anie.202519836","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T09:25:21Z","timestamp":1774862721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Single\u2010Injection Multi\u2010Omics Analysis by Direct Infusion Mass Spectrometry"],"prefix":"10.1002","volume":"65","author":[{"given":"Yuming","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Computational Biomedicine Cedars Sinai Medical Center  Los Angeles California USA"},{"name":"Advanced Clinical Biosystems Research Institute Cedars Sinai Medical Center  Los Angeles California USA"},{"name":"Smidt Heart Institute Cedars Sinai Medical Center  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Salladay\u2010Perez","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology Immunology, and Molecular Genetics University of California  Los Angeles USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Momenzadeh","sequence":"additional","affiliation":[{"name":"Department of Computational Biomedicine Cedars Sinai Medical Center  Los Angeles California USA"},{"name":"Advanced Clinical Biosystems Research Institute Cedars Sinai Medical Center  Los Angeles California USA"},{"name":"Smidt Heart Institute Cedars Sinai Medical Center  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Mu\u00f1oz\u2010Estrada","sequence":"additional","affiliation":[{"name":"Department of Computational Biomedicine Cedars Sinai Medical Center  Los Angeles California USA"},{"name":"Advanced Clinical 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