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We report the label\u2010free imaging of microobjects and nanoobjects with a sharp negative optical contrast. The shadow ECL images obtained are the optical signatures of the blocking of the electron\u2010transfer reaction convoluted by the chemical reactivity and local diffusional hindrance of the ECL reagents by the object deposited on the electrode. Here, we address first the critical aspect of the smallest spherical nanoparticle imaged by shadow ECL microscopy. By investigating the imaging limits of shadow ECL, we demonstrate its capability to image single nanoparticles down to 50\u00a0nm. This work uncovers the sensitivity of the method by not only showing the smallest particles imaged by shadow ECL to date, but also, counterintuitively, a contrastive behavior of the single insulating particles with increased electrochemical rates at the insulator\u2010electrolyte\u2010electrode triple point. Finally, the practical utility of this shadow ECL method is illustrated by imaging complex samples such as densely packed particle assemblies or microbial\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    spores. It also opens new avenues for single\u2010molecule and shadow ECL nano imaging of catalytic and biological systems.\n                  <\/jats:p>","DOI":"10.1002\/anie.202521654","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T06:19:41Z","timestamp":1773641981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["From Microscale to Nanoscale Shadow Electrochemiluminescence Microscopy"],"prefix":"10.1002","volume":"65","author":[{"given":"Xiaodan","family":"Gou","sequence":"first","affiliation":[{"name":"University of Bordeaux Bordeaux INP ISM  Pessac France"},{"name":"State Key Laboratory of Analytical Chemistry For Life Science School of Chemistry and Chemical Engineering Nanjing University  Nanjing P. R. 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