{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:23:00Z","timestamp":1791300180418,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YFA0921900"],"award-info":[{"award-number":["2025YFA0921900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC 22378305"],"award-info":[{"award-number":["NSFC 22378305"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,4,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Microbial electrosynthesis (MES) systems aim to use electroactive microorganisms (EAMs) to achieve electricity\u2010driven CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation for biosynthesis of multicarbon chemicals. However, the low efficiencies of extracellular electron transfer (EET) and CO\n                    <jats:sub>2<\/jats:sub>\n                    assimilation of EAMs remain the essential limiting factors that restrict performance of MES systems. Herein, we developed an electrosynthetic biohybrid system to synergistically supply electrons and CO\n                    <jats:sub>2<\/jats:sub>\n                    to\n                    <jats:italic>Rhodopseudomonas palustris<\/jats:italic>\n                    (an EAM) for lycopene biosynthesis. Intracellular carbon and energy fluxes were redirected by strengthening the lycopene biosynthesis pathway and blocking the nitrogen\u2010fixation pathway, resulting in 23\u2010fold increase in lycopene yield than that of the wild\u2010type\n                    <jats:italic>R. palustris<\/jats:italic>\n                    . To enhance extracellular transfer of CO\n                    <jats:sub>2<\/jats:sub>\n                    and electrons to\n                    <jats:italic>R. palustris<\/jats:italic>\n                    , metal\u2010organic frameworks (MOFs) with high CO\n                    <jats:sub>2<\/jats:sub>\n                    adsorption capacity were assembled with polydopamine on cell membrane to construct a biohybrid MES system, which produced 3.55\u00a0mg\/L lycopene in two consecutive MES cycles, the highest lycopene production from CO\n                    <jats:sub>2<\/jats:sub>\n                    . Electrochemical and transcriptomic analyses revealed that the biohybrid MES system stimulated microbial metabolism including EET, the Calvin\u2010Benson\u2010Bassham cycle and lycopene biosynthesis, thereby improving CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010to\u2010chemical conversion. This study demonstrated directional supply of electrons and CO\n                    <jats:sub>2<\/jats:sub>\n                    to EAMs enabled high\u2010performance MES systems, which also offered insights into the mechanisms underlying efficient CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation and carbon\u2010negative biomanufacturing.\n                  <\/jats:p>","DOI":"10.1002\/anie.202522703","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:55:05Z","timestamp":1772996105000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Microbial Electrosynthetic Biohybrid System to Synergistically Supply Electrons and CO\n                    <sub>2<\/sub>\n                    to\n                    <i>Rhodopseudomonas palustris<\/i>\n                    for Lycopene Production"],"prefix":"10.1002","volume":"65","author":[{"given":"Bo","family":"Xiong","sequence":"first","affiliation":[{"name":"State Key Laboratory of Synthetic Biology Tianjin University  Tianjin China"},{"name":"School of Synthetic Biology and Biomanufacturing Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Synthetic Biology Tianjin University  Tianjin China"},{"name":"School of Synthetic Biology and Biomanufacturing Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Synthetic Biology Tianjin University  Tianjin China"},{"name":"School of Synthetic Biology and Biomanufacturing Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianrui","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Synthetic Biology Tianjin University  Tianjin China"},{"name":"School of Synthetic Biology and Biomanufacturing Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Life and Health Sciences Northeastern University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0447-3617","authenticated-orcid":false,"given":"Hao","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Synthetic Biology Tianjin University  Tianjin China"},{"name":"School of Synthetic Biology and Biomanufacturing Tianjin University  Tianjin China"},{"name":"College of Life and Health Sciences Northeastern University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adg2495"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010025\u201008751\u20103"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1248365"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201043524\u20104"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1706371114"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2020.10.014"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5039"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2023.147152"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202407599"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joule.2020.03.001"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1217643"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.8b00226"},{"key":"e_1_2_7_14_1","volume-title":"Lange's Handbook of Chemistry","author":"Dean J. A.","year":"1998"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2025.108632"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41893\u2010023\u201001233\u20102"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929\u2010022\u201000867\u20103"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929\u2010024\u201001198\u20101"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929\u2010022\u201000817\u2010z"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad3317"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat9777"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202305189"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201009377\u20106"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400641x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joule.2024.10.005"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2GC02467E"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2020.08.007"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02668\u201019"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2023.08.004"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202412072"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apcatb.2024.123946"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8GC03417F"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2210538119"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.187.22.7784\u20107794.2005"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf2091"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.0c00496"},{"key":"e_1_2_7_37_1","doi-asserted-by":"crossref","unstructured":"J. F.Ortiz\u2010Medina M. R.Poole A. M.Grunden andD. F.Call \u201cNitrogen Fixation and Ammonium Assimilation Pathway Expression ofGeobacter SulfurreducensChanges in Response to the Anode Potential in Microbial Electrochemical Cells \u201dApplied and Environmental Microbiology89(2023): e02073\u201302022 https:\/\/doi.org\/10.1128\/aem.02073\u201022.","DOI":"10.1128\/aem.02073-22"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602439103"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.1c00539"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijhydene.2019.04.050"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40820\u2010015\u20100040\u2010x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12274\u2010023\u20106396\u20101"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12274\u2010023\u20106398\u2010z"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9CC03847G"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2023.108170"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa4834"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwac128"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202522703","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.202522703","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202522703","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T04:39:23Z","timestamp":1775536763000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.202522703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,27]]},"references-count":46,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2026,4,6]]}},"alternative-id":["10.1002\/anie.202522703"],"URL":"https:\/\/doi.org\/10.1002\/anie.202522703","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,27]]},"assertion":[{"value":"2025-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22703"}}