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Here, we introduce a protocol to detect structural water molecules under native conditions, using long\u2010lived hyperpolarized water (HyperW) enabled by UV\u2010induced, nonpersistent radicals. In the model protein chymotrypsin inhibitor 2, HyperW\u2010enhanced two\u2010dimensional NMR correlation spectra across pD 5.5\u20138.4 reveal strong exchange\u2010driven enhancements at solvent\u2010exposed residues. By contrast, a distinct group of four residues shows hyperpolarized amide signals, which disappear when through\u2010space polarization transfer via nuclear Overhauser effect (NOE) is suppressed using a CLEANEX\u2010PM experiment. The CLEANEX\u2010negative\/HyperW\u2010positive signature, together with the spatial proximity of these residues to crystallographically conserved water molecules, supports NOE\u2010mediated transfer from long\u2010residence internal water, not distinguishable by standard NMR methods. The combined observables establish HyperW NMR as a residue\u2010specific reporter of structural hydration and hydration\u2010coupled dynamics under native conditions, providing a route to link conserved water observed in crystals to their roles in\u00a0solution.<\/jats:p>","DOI":"10.1002\/anie.202523739","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:06:07Z","timestamp":1773000367000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Residue\u2010Specific Signatures of Structural Water Identified by Dissolution Dynamic Nuclear Polarization with UV\u2010Generated Radicals"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7262-5497","authenticated-orcid":false,"given":"Fabian","family":"Hecker","sequence":"first","affiliation":[{"name":"Department of Health Technology Technical University of Denmark (DTU) Kongens Lyngby 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