{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:01:43Z","timestamp":1790812903378,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:00:00Z","timestamp":1771632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:00:00Z","timestamp":1771632000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,3,27]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Copper dysregulation is implicated in neurodegenerative diseases such as Alzheimer's disease (AD), yet its precise role in neuronal death remains unclear. To address this issue, here, we introduce a pair of Cu\n                    <jats:sup>+<\/jats:sup>\n                    and Cu\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    specific DNAzymes\u2010based fluorescent probes, for the first time, enabling simultaneous visualization of both redox states of copper in single living neurons. Using this dual\u2010color system, we found that amyloid\u2010beta (A\u03b2) oligomerization promotes intracellular copper accumulation, distinct from that induced by artificial ionophore loading. Elevated Cu\n                    <jats:sup>+<\/jats:sup>\n                    drives reactive oxygen species (ROS) generation, lipoylated protein aggregation, and FDX1\u2010dependent cuproptosis, while Cu\n                    <jats:sup>+<\/jats:sup>\n                    chelation or FDX1 knockdown completely prevents cell death. In contrast, ROS scavengers only partially rescue viability, demonstrating that neuronal death is driven by copper overload, not oxidative stress itself. These findings redefine the mechanistic framework linking copper redox imbalance to A\u03b2 pathology and neuronal vulnerability and demonstrate a selective, sensitive approach for monitoring copper homeostasis and its disruption in neurodegenerative disease.\n                  <\/jats:p>","DOI":"10.1002\/anie.202524237","type":"journal-article","created":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T09:03:37Z","timestamp":1771664617000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Simultaneous Imaging of Cu\n                    <sup>+<\/sup>\n                    and Cu\n                    <sup>2<\/sup>\n                    <sup>+<\/sup>\n                    in Neural Cells Using DNAzyme Probes Reveals Mechanistic Link Between Copper Redox Imbalance and Amyloid Pathology"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8118-6414","authenticated-orcid":false,"given":"Xiangli","family":"Shao","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Texas at Austin  Austin Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2743-5779","authenticated-orcid":false,"given":"Zhenglin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Texas at Austin  Austin Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5694-2142","authenticated-orcid":false,"given":"Weijie","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences University of Texas at Austin  Austin Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1203-6441","authenticated-orcid":false,"given":"Whitney","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Texas at Austin  Austin Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyi","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"The University of Texas MD Anderson Cancer Center, UT Health Graduate School of Biomedical Sciences  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1221-6709","authenticated-orcid":false,"given":"Yi","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Texas at Austin  Austin Texas USA"},{"name":"Department of Molecular Biosciences University of Texas at Austin  Austin Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2020.12.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010024\u201007206\u20103"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010023\u201004533\u20105"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422\u2010022\u201000653\u20107"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf0529"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010022\u201001229\u2010y"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775\u2010009\u20100600\u2010y"},{"key":"e_1_2_8_9_1","first-page":"323","article-title":"Iron and Copper Toxicity in Diseases of Aging, Particularly Atherosclerosis and Alzheimer's Disease","volume":"232","author":"Brewer G. 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